Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of human cutaneous tumors to identify gene expression patterns involved in the malignant transformation and progression of metastatic melanoma

ABSTRACT: Metastatic melanoma is a deadly disease while non-metastatic melanoma and other cutaneous tumor types are usually cured with surgical removal of the primary tumors. This study evaluated gene expresion to determine if gene expression differences existed which would allow one to identify the metastatic tumors based on the expression of specific genes. Experiment Overall Design: Tumor samples were collected from patients with various cutaneous tumors, flash frozen, and then processed for gene exprression analysis using microarrays. The pool of tissues includes enough metastatic samples and non-metastatic samples to identify a gene expression signature associated with the outgrowth of the metastatic tumor.

ORGANISM(S): Homo sapiens

SUBMITTER: Sean Yoder

PROVIDER: E-GEOD-7553 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The gene expression profiles of primary and metastatic melanoma yields a transition point of tumor progression and metastasis.

Riker Adam I AI Enkemann Steven A SA Fodstad Oystein O Liu Suhu S Ren Suping S Morris Christopher C Xi Yaguang Y Howell Paul P Metge Brandon B Samant Rajeev S RS Shevde Lalita A LA Li Wenbin W Eschrich Steven S Daud Adil A Ju Jingfang J Matta Jaime J

BMC medical genomics 20080428

<h4>Background</h4>The process of malignant transformation, progression and metastasis of melanoma is poorly understood. Gene expression profiling of human cancer has allowed for a unique insight into the genes that are involved in these processes. Thus, we have attempted to utilize this approach through the analysis of a series of primary, non-metastatic cutaneous tumors and metastatic melanoma samples.<h4>Methods</h4>We have utilized gene microarray analysis and a variety of molecular techniqu ...[more]

PMID: 18442402

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Project description:The present study deals with functional interactions of cutaneous and brain-metastasizing human melanoma cells with brain-derived molecules. In this study we employed the unique melanoma xenograft model developed by Izraely and described in Int J Cancer. 2011 Oct 25. doi: 10.1002/ijc.27324. The present study aims to determine if brain-derived soluble factors regulate malignancy-associated functions of cutaneous and brain-metastasizing melanoma cells and identify which functions are regulated by such factors. The working hypothesis of this study is that the interactions between the brain microenvironment and melanoma cells determine metastasis formation at this organ site. The aim of the study was to evaluate the contribution of such interactions to the formation of brain metastasis in nude mice xenografted with human melanoma cells. An insight into these interactions is an essential pre-requisite for the development of effective targeted therapy for melanoma brain metastasis. We assessed the effects of soluble factors present in supernatants of short-term cultures of normal mouse brain (referred here after as brain-derived soluble factors) on several characteristics linked to melanoma brain metastasis. It was found that brain-derived soluble factors affect differentially cutaneous and brain-metastasizing melanoma cells variants in-vitro. Such factors enhanced the viability of cutaneous melanoma cells but caused an S phase arrest followed by apoptosis of brain-metastasizing cells. Brain-derived soluble factors enhanced migration of melanoma cells metastasizing to the brain, but did not affect the migration of the cutaneous variants. Such factors up-regulated the expression of the chemokine receptor CCR4 in both cutaneous and brain metastasizing melanoma cells. It is not unlikely that CCR4 ligands expressed in the brain interact with the CCR4-expressing melanoma cells thereby directing them to the brain. Brain-derived soluble factors enhanced the transmigration, across human brain endothelial cells of cutaneous but not of brain metastasizing melanoma variants. This activity could promote the capacity of the cutaneous cells to metastasize to the brain. 4 Samples (arrays) were analyzed. There is 1 replicate for each variant and each treatment. We generated pairwise comparisons between cutaneous and brain metastatic variants of the same genetic background, using Partek Genomics Suite, in the three melanoma models. Genes with p≤5% and a fold-change difference of ≥2 or <-2 were selected.

Dataset Information

Transcription profiling of human cutaneous tumors to identify gene expression patterns involved in the malignant transformation and progression of metastatic melanoma

Publications

The gene expression profiles of primary and metastatic melanoma yields a transition point of tumor progression and metastasis.

Similar Datasets

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