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Transcription profiling of human synovial fibroblasts from rheumatoid arthritis and osteoarthritis patients

ABSTRACT: mRNA expression levels in synovial fibroblasts in 6 rheumatoid arthritis patients versus 6 osteoarthritis patients. Experiment Overall Design: Synovial tissue was obtained from open joint replacement surgery or Experiment Overall Design: arthroscopic synovectomy. Patients with RA or OA (n = 6 each for gene expression analysis and further patients for validation experiments) were classified according to the ACR criteria.

ORGANISM(S): Homo sapiens

DISEASE(S): rheumatoid arthritis

SUBMITTER: Andreas Beyer

PROVIDER: E-GEOD-7669 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Constitutive upregulation of the transforming growth factor-beta pathway in rheumatoid arthritis synovial fibroblasts.

Pohlers Dirk D Beyer Andreas A Koczan Dirk D Wilhelm Thomas T Thiesen Hans-Jürgen HJ Kinne Raimund W RW

Arthritis research & therapy 20070101 3

Genome-wide gene expression was comparatively investigated in early-passage rheumatoid arthritis (RA) and osteoarthritis (OA) synovial fibroblasts (SFBs; n = 6 each) using oligonucleotide microarrays; mRNA/protein data were validated by quantitative PCR (qPCR) and western blotting and immunohistochemistry, respectively. Gene set enrichment analysis (GSEA) of the microarray data suggested constitutive upregulation of components of the transforming growth factor (TGF)-beta pathway in RA SFBs, with ...[more]

PMID: 17594488

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