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An essential role for UTX in resolution and activation of bivalent promoters

ABSTRACT: In the present study, we show that UTX plays an essential role in resolving and activating many retinoic acid (RA)-inducible bivalent genes during the RA-driven differentiation of mouse ESCs treated with a physiologically relevant RA concentration (0.2 Î¼M). We showed that UTX loss and UTX knockdown interfered with the RA-induced differentiation of mouse ESCs. Therefore, our findings indicate that the UTX-mediated resolution and activation of many RA-inducible bivalent genes, including numerous Hoxa-d cluster genes, are required for RA-driven differentiation of mouse ESCs. ChIP-Seq data for H3K4me3 and H3K27me3 were generated along with input data for mouse stem cells with wild type or UTX-depleted genotype and with or without RA treatment.

ORGANISM(S): Mus musculus

SUBMITTER: Alin Tomoiaga

PROVIDER: E-GEOD-76692 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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An essential role for UTX in resolution and activation of bivalent promoters.

Dhar Shilpa S SS Lee Sung-Hun SH Chen Kaifu K Zhu Guangjing G Oh WonKyung W Allton Kendra K Gafni Ohad O Kim Young Zoon YZ Tomoiga Alin S AS Barton Michelle Craig MC Hanna Jacob H JH Wang Zhibin Z Li Wei W Lee Min Gyu MG

Nucleic acids research 20160113 8

Trimethylated histone H3 lysine 27 (H3K27me3) is linked to gene silencing, whereas H3K4me3 is associated with gene activation. These two marks frequently co-occupy gene promoters, forming bivalent domains. Bivalency signifies repressed but activatable states of gene expression and can be resolved to active, H3K4me3-prevalent states during multiple cellular processes, including differentiation, development and epithelial mesenchymal transition. However, the molecular mechanism underlying bivalenc ...[more]

PMID: 26762983

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An essential role for UTX in resolution and activation of bivalent promoters

Project description:In the present study, we show that UTX plays an essential role in resolving and activating many retinoic acid (RA)-inducible bivalent genes during the RA-driven differentiation of mouse ESCs treated with a physiologically relevant RA concentration (0.2 μM). We showed that UTX loss and UTX knockdown interfered with the RA-induced differentiation of mouse ESCs. Therefore, our findings indicate that the UTX-mediated resolution and activation of many RA-inducible bivalent genes, including numerous Hoxa-d cluster genes, are required for RA-driven differentiation of mouse ESCs.

2016-02-05 | GSE76692 | GEO

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2015-03-01 | E-GEOD-65089 | biostudies-arrayexpress

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