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FOXA and master transcription factors recruit Mediator and Cohesin to the core transcriptional regulatory circuitry of cancer cells

ABSTRACT: The Mediator and Cohesin complexes control the cellular transcriptional program. However, it is not well understood how they are recruited to regulatory regions. Our study shows that the core transcriptional regulatory circuitry of cancer cells is essential to recruit Mediator, Cohesin and NIPBL to enhancer and promoter regions of actively transcribed genes in cancer cells. ChIP-seq analysis of Mediator (MED1), Cohesin (SMC1A) and NIPBL in HEPG2, A549 and MCF7 cells. The analysis also includes ChIP-Seq data for the transcription factor ERa in MCF7 cells treated with estradiol.

ORGANISM(S): Homo sapiens

SUBMITTER: Steve Bilodeau

PROVIDER: E-GEOD-76893 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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FOXA and master transcription factors recruit Mediator and Cohesin to the core transcriptional regulatory circuitry of cancer cells.

Fournier Michèle M Bourriquen Gaëlle G Lamaze Fabien C FC Côté Maxime C MC Fournier Éric É Joly-Beauparlant Charles C Caron Vicky V Gobeil Stéphane S Droit Arnaud A Bilodeau Steve S

Scientific reports 20161014

Controlling the transcriptional program is essential to maintain the identity and the biological functions of a cell. The Mediator and Cohesin complexes have been established as central cofactors controlling the transcriptional program in normal cells. However, the distribution, recruitment and importance of these complexes in cancer cells have not been fully investigated. Here we show that FOXA and master transcription factors are part of the core transcriptional regulatory circuitry of cancer ...[more]

PMID: 27739523

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