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Transcription profiling of mouse how germ-line clones embryos and WT embryos at 3-5 h AEL

ABSTRACT: Mutant embryos lacking maternal and zygotic HOW exhibit defects in mesoderm development. How is an RNA binding protein that regulates the levels of mRNAs by controling RNA metabolism. To gain an insight as to the profile of mRNAs regulated by HOW during early mesoderm development we analyzed the array of mRNAs whose levels are altered in the how mutant embryos. Experiment Overall Design: Embryos lacking both maternal and zygoic HOW were selected using a GFP balancer. The mRNA of 5 collections of 3-5 hrs old WT or how mutant embryos were pooled and used for each microarray experiment. Two microarray experiments were performed from independent samples.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Talila Volk

PROVIDER: E-GEOD-7772 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Post-transcriptional repression of the Drosophila midkine and pleiotrophin homolog miple by HOW is essential for correct mesoderm spreading.

Toledano-Katchalski Hila H Nir Ronit R Volohonsky Gloria G Volk Talila T

Development (Cambridge, England) 20070829 19

The even spreading of mesoderm cells in the Drosophila embryo is essential for its proper patterning by ectodermally derived signals. In how germline clone embryos, defects in mesoderm spreading lead to a partial loss of dorsal mesoderm derivatives. HOW is an RNA-binding protein that is thought to regulate diverse mRNA targets. To identify direct HOW targets, we implemented a series of selection methods on mRNAs whose levels were elevated in how germline clone embryos during the stage of mesoder ...[more]

PMID: 17728343

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Protein interactors of MEG-1 in C. elegans embryos

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2022-10-05 | PXD036610 | Pride

Transcription profiling of Drosophila mef2 allelic series reveals mef2 activity levels differentially affect gene expression during muscle development

Project description:The conserved Mef2 transcription factor is a major regulator of gene expression and differentiation. Recent genomic studies have identified a large number of mef2-regulated target genes with distinct temporal expression profiles during Drosophila myogenesis. However, the question remains as to how a single transcription factor can control such diverse patterns of gene expression. The aim of this project was to investigate whether there are genes with different mef2-requirements for their expression during muscle differentiation in vivo during the development of Drosophila melanogaster. Experiment Overall Design: We used microarrays in conjunction with a mef2 allelic series to determine the gene expression profile in developing embryos at five different levels of mef2 activity. The allelic series extends from a null mutation, mef222.21, through to the control, via three hypomorphic alleles, mef2113, mef2424, and mef265. The series corresponds to different levels of Mef2 in the following order: control > 65 > 424 > 113 > 22.21. The control stock for the microarray analysis was dp cn a px sp, the stock used for the mutagenesis that produced mef265, mef2424 and mef2113. For the microarrays, 30 minute collections of control and mutant embryos for each mef2 allele were individually staged and pools of 150 embryos were then processed at mid stage 13. This corresponds to the early differentiation phase of muscle development and the expression of multiple muscle sarcomeric protein genes. Quadruplicate samples were assayed using AffymetrixÂ® Genechips by the Flychip Drosophila microarray resource.

2008-06-17 | E-GEOD-9889 | biostudies-arrayexpress

Comparison between mRNAs of how germ-line clones embryos and WT embryos at 3-5 h AEL

Project description:Mutant embryos lacking maternal and zygotic HOW exhibit defects in mesoderm development. How is an RNA binding protein that regulates the levels of mRNAs by controling RNA metabolism. To gain an insight as to the profile of mRNAs regulated by HOW during early mesoderm development we analyzed the array of mRNAs whose levels are altered in the how mutant embryos. Keywords: Genetic modification

2007-09-24 | GSE7772 | GEO

Precise temporal regulation of post-transcriptional repressors is required for an orderly Drosophila maternal-to-zygotic transition

Project description:In animal embryos the maternal-to-zygotic transition (MZT) hands developmental control from maternal to zygotic gene products. We show that the maternal proteome represents over half of the protein coding capacity of the Drosophila melanogaster genome and that 2% of this proteome is rapidly degraded during the MZT. Cleared proteins include the post-transcriptional repressors Cup, Trailer hitch (TRAL), Maternal expression at 31B (ME31B), and Smaug (SMG). While the ubiquitin-proteasome system is necessary for clearance of all four repressors, distinct E3 ligase complexes target them: the C-terminal to Lis1 Homology (CTLH) complex targets Cup, TRAL and ME31B for degradation early in the MZT; the Skp/Cullin/F-box-containing (SCF) complex targets SMG at the end of the MZT. Deleting the C-terminal 233 amino acids of SMG make it immune to degradation. We show that artificially persistent SMG downregulates the zygotic re-expression of mRNAs whose maternal contribution is cleared by SMG. Thus, clearance of SMG permits an orderly MZT.

2020-06-05 | PXD016523 | Pride

Single-cell chromatin accessibility profiling of mesoderm development in wild-type and mutant embryos

Project description:Dissection of the functional input of transcription factors in embryos at a molecular and cellular level by sci-ATAC-seq profiling wild-type and TF mutant embryos on a time course of mesoderm/muscle development.

2022-01-18 | E-MTAB-10489 | biostudies-arrayexpress

Identification of Endos interacting proteins in Drosophila embryos by immunoprecipitation and LC-MS-MS/MS analysis

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2024-08-09 | PXD036613 | Pride

Transcription profiling of zebrafish embryos injected with DrC1q-like morpholino and control morpholino

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Expression data from early Zebrafish embryos after knockdown of mir-34

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2013-04-03 | E-GEOD-32360 | biostudies-arrayexpress

Protein expression profiling in eggs and embryos from control and Eif4e1b knockout female mice.

Project description:We have identified eukaryotic translation initiation factor 4E family member 1B (Eif4e1b) as a regulator of maternal RNA translation, whose maternal deletion leads to 2-cell arrest phenotype in mouse embryos. eIF4E1b protein binds mRNA selectively in oocytes and controls their translation in early embryos. We aim to profile the global changes in protein expression in metaphase II (MII) eggs and zygotes collected from control or Eif4e1b knockout female mice. An encapsulated proteomic-sample processing protocol is used to perform this low-input mass spectrometry to quantify proteomic changes (Kulak et al. 2014/PMID 24497582).

2023-06-12 | PXD041583 | Pride

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