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Detection of genes in different carbohydrates isolated by NSH using single channel slide based hybrdizations

ABSTRACT: Isolation of genes from Aspergillus nidulans grown in different complex carbohydrates by NSH and the NSH method was evaluated by using single channel non competitive slide based hybridizations Keywords = NSH Keywords = subtraction Keywords = aspergillus Keywords = polysaccharide Keywords = exopolygalacturonase

ORGANISM(S): Emericella nidulans

SUBMITTER: Rolf Prade

PROVIDER: E-GEOD-783 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Negative subtraction hybridization: an efficient method to isolate large numbers of condition-specific cDNAs.

Ray Anamika A Macwana Sunita S Ayoubi Patricia P Hall Leo T LT Prade Rolf R Mort Andrew J AJ

BMC genomics 20040329 1

<h4>Background</h4>The construction of cDNA libraries is a useful tool to understand gene expression in organisms under different conditions, but random sequencing of unbiased cDNA collections is laborious and can give rise to redundant EST collections. We aimed to isolate cDNAs of messages induced by switching Aspergillus nidulans from growth on glucose to growth on selected polysaccharides. Approximately 4,700 contigs from 12,320 ESTs were already available from a cDNA library representing tra ...[more]

PMID: 15050035

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Detection of genes in different carbohydrates isolated by NSH using single channel slide based hybrdizations

Project description:Isolation of genes from Aspergillus nidulans grown in different complex carbohydrates by NSH and the NSH method was evaluated by using single channel non competitive slide based hybridizations Keywords = NSH Keywords = subtraction Keywords = aspergillus Keywords = polysaccharide Keywords = exopolygalacturonase Keywords: repeat sample

2004-03-14 | GSE783 | GEO

A tri-species Aspergillus array

Project description:This SuperSeries is composed of the following subset Series: GSE9275: A tri-species Aspergillus array (nidulans arrays) GSE9276: A tri-species Aspergillus array (niger arrays) GSE9277: A tri-species Aspergillus array (oryzae arrays) Keywords: SuperSeries Refer to individual Series

2008-02-10 | E-GEOD-9298 | biostudies-arrayexpress

Expression analysis of Aspergillus nidulans OE::rsmA

Project description:Investigation of whole genome gene expression level changes in Aspergillus nidulans OE::rsmA compared to wild-type RDIT9.32 (veA). A twelve array study using total RNA recovered from six separate cultures of Aspergillus nidulans wild-type RDIT9.32 (veA) and six separate cultures of Aspergillus nidulans overexpressing rsmA (restorer of secondary metabolism A), using custom-designed, four-plex arrays. The experiment was divided into two runs. In the first run, three biological replicates each of Aspergillus nidulans wild-type RDIT9.32 (veA) and Aspergillus nidulans carrying a plasmid overexpressing rsmA under the control of the gpdA promoter were assayed. In the second run, three biological replicates each of Aspergillus nidulans wild-type RDIT9.32 (veA) and Aspergillus nidulans overexpressing rsmA at the native locus under the control of the gpdA promoter were assayed.

2012-01-17 | E-GEOD-34220 | biostudies-arrayexpress

Expression analysis of Aspergillus nidulans AN1599 (PbcR) overexpression mutant

Project description:Investigation of whole genome gene expression level changes in Aspergillus nidulans AN1599 (PbcR) overexpression mutant, compared to the FGSC A4 wild-type strain. Overexpression of the Zn(II)2Cys6 –type transcription factor, AN1599.4 (PbcR, pimaradiene biosynthetic cluster regulator), activates a secondary metabolite gene cluster in Aspergillus nidulans. Activation of the pathway in Aspergillus nidulans lead to a production of ent-pimara-8(14),15-diene.

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Carbon starvation and pectin utilization in Aspergillus nidulans

Project description:We have a cDNA microarray to investigate changes in gene expression following transfer of fungal cultures from growth on glucose to growth on pectin or no carbon source. Our goal was to asses the roles of release from carbon catabolite repression and specific induction on proteins needed for metabolism (or utilization) of a single class of complex polysaccharide. Keywords = Aspergillus Keywords = Pectin Keywords = central metabolism Keywords = pectin Keywords = carbon catabolite repression Keywords = polysaccharide Keywords = exopolygalacturonase

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Carbon starvation and pectin utilization in Aspergillus nidulans

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Transcriptome changes initiated by carbon starvation in Aspergillus nidulans (Microbiology-SGM, 2013, Vol:159)

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Gene expression in Aspergillus nidulans on multiple nitrogen sources

Project description:Stranded RNA-seq of wild type Aspergillus nidulans during growth on a range of nitrogn sources

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The role of carbon starvation in the induction of enzymes that degrade plant-derived carbohydrates in Aspergillus niger

Project description:Fungi are an important source of enzymes for saccharification of plant polysaccharides and production of biofuels. Understanding of the regulation and induction of expression of genes encoding these enzymes is still incomplete. To explore the induction mechanism, we analysed the response of the industrially important fungus Aspergillus niger to wheat straw, with a focus on events occurring shortly after exposure to the substrate. RNA sequencing showed that over a third of the genes induced after 6 h of exposure to wheat straw were also induced during 6 h of carbon starvation, indicating that carbon starvation is probably an important factor in the early response to wheat straw. The up-regulation of the expression of a high number of genes encoding CAZymes that are active on plant-derived carbohydrates during early carbon starvation suggests that these enzymes could be involved in a scouting role during starvation, releasing inducing sugars from complex plant polysaccharides. Eight samples in total consisting of duplicate shake flask Aspergillus niger cultures from four conditions: 48h glucose, 6 h starvation, 6 h wheat straw, 24 h starvation

2014-08-22 | E-GEOD-57315 | biostudies-arrayexpress

Expression analysis of Aspergillus nidulans OE::rsmA

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