Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Analysis of melanosomal, exosomal and cellular miRNAs of melanoma cells


ABSTRACT: miRNA microarray profiling was performed for exosomes and secreted melanosomes of MNT-1 cells as well as WM3682 and WM3314 cells and melanosomes isolated from cell homogenates. The aim of this study is to compare the miRNA content of exosomes and secreted melanosomes and to assess differences in the miRNA profiles of different melanoma cell lines and their melanosomes. Total RNA was extracted from MNT-1 exosomes and secreted melanosomes (2 replicates each) that were isolated from the conditioned medium of MNT-1 melanoma cells by differential ultracentrifugation. In addition, total RNA was isolated from WM3682 and WM3314 melanoma cells and melanosomes (1 replicate each). These latter melanosomes were isolated from cell homogenates by filtration through a 0.45 µm filter, followed by FACS sorting. Contributor: Microarray Unit of the Core Facility Genomics and Proteomics DKFZ

ORGANISM(S): Homo sapiens

SUBMITTER: Carmit Levy 

PROVIDER: E-GEOD-79411 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2016-08-09 | E-GEOD-72337 | biostudies-arrayexpress
2016-08-09 | E-GEOD-72620 | biostudies-arrayexpress
2016-08-09 | E-GEOD-72229 | biostudies-arrayexpress
2013-09-27 | E-GEOD-49298 | biostudies-arrayexpress
2014-07-02 | E-GEOD-39877 | biostudies-arrayexpress
2011-06-30 | E-GEOD-22692 | biostudies-arrayexpress
2014-01-30 | E-GEOD-54493 | biostudies-arrayexpress
2020-05-27 | PXD017421 | Pride
2013-03-28 | E-GEOD-45532 | biostudies-arrayexpress
2014-07-01 | E-GEOD-49144 | biostudies-arrayexpress