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The histone H3K9 demethylase KDM3A promotes anoikis through transcriptional activation of pro-apoptotic genes BNIP3 and BNIP3L

ABSTRACT: We isolated and sequenced the shRNA region of a pSM2 retroviral plasmid to identify which shRNAs were enriched in MCF10A cells grown in detached conditions versus the same MCF10A cells that were grown under normal, attached conditions. Comparison of the ratio of shRNA sequences between cells grown under normal conditions versus cells grown in detached conditions.

ORGANISM(S): synthetic construct

SUBMITTER: Victoria Pedanou

PROVIDER: E-GEOD-80144 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The histone H3K9 demethylase KDM3A promotes anoikis through transcriptional activation of pro-apoptotic genes BNIP3 and BNIP3L

Project description:We isolated and sequenced the shRNA region of a pSM2 retroviral plasmid to identify which shRNAs were enriched in MCF10A cells grown in detached conditions versus the same MCF10A cells that were grown under normal, attached conditions.

2016-07-29 | GSE80144 | GEO

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Control shRNA- vs. obscurin shRNA-expressing MCF10A cells

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Genome-wide analysis of gene expression perturbed by Pontin shRNA in MCF7 subjected to normoxic and hypoxic conditions

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