Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of Coturnix japonica during the photoinduction process.

ABSTRACT: The molecular mechanism of photoperiodic time measurement remains unknown in animals. Japanese quail is an excellent model for studying these phenomena because of their rapid and dramatic response to photoperiod. When quail are transferred from short to long day conditions, increase in plasma gonadotrophin (luteinising hormone: LH) can be observed by the end of the first long day and this phenomenon is called âfirst day release modelâ. To dissect the system dynamics and network structure regulating photoperiodism, we examined global gene expression using high-density oligonucleotide microarray for chicken (Affymetrix Chicken Genome Array). Experiment Overall Design: The mediobasal hypothalamus (MBH) of quail was collected from 6 birds for each time point during the photoinduction process. Pooled total RNA from three MBHs was labelled and hybridized to each Affymetrix chicken genome array. Samples were analyzed in duplicate set of array (two biological replicate).

ORGANISM(S): Coturnix japonica

SUBMITTER: Takashi Yoshimura

PROVIDER: E-GEOD-8017 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Thyrotrophin in the pars tuberalis triggers photoperiodic response.

Nakao Nobuhiro N Ono Hiroko H Yamamura Takashi T Anraku Tsubasa T Takagi Tsuyoshi T Higashi Kumiko K Yasuo Shinobu S Katou Yasuhiro Y Kageyama Saburo S Uno Yumiko Y Kasukawa Takeya T Iigo Masayuki M Sharp Peter J PJ Iwasawa Atsushi A Suzuki Yutaka Y Sugano Sumio S Niimi Teruyuki T Mizutani Makoto M Namikawa Takao T Ebihara Shizufumi S Ueda Hiroki R HR Yoshimura Takashi T

Nature 20080301 7185

Molecular mechanisms regulating animal seasonal breeding in response to changing photoperiod are not well understood. Rapid induction of gene expression of thyroid-hormone-activating enzyme (type 2 deiodinase, DIO2) in the mediobasal hypothalamus (MBH) of the Japanese quail (Coturnix japonica) is the earliest event yet recorded in the photoperiodic signal transduction pathway. Here we show cascades of gene expression in the quail MBH associated with the initiation of photoinduced secretion of lu ...[more]

PMID: 18354476

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Project description:Living organisms detect seasonal changes in day length (photoperiod), and alter their physiological functions accordingly, to fit seasonal environmental changes. This photoperiodic system is implicated in seasonal affective disorders and the season-associated symptoms observed in bipolar disease and schizophrenia. Thyroid-stimulating hormone beta subunit (Tshb), induced in the pars tuberalis (PT), plays a key role in the pathway that regulates animal photoperiodism. However, the upstream inducers of Tshb expression remain unknown. Here we show that late-night light stimulation acutely triggers the Eya3-Six1 pathway, which directly induces Tshb expression. Using melatonin-proficient CBA/N mice, which preserve the photoperiodic Tshb-expression response, we performed a genome-wide expression analysis of the PT under chronic short-day and long-day conditions. These data comprehensively identified long-day and short-day genes, and indicated that late-night light stimulation induces long-day genes. We verified this by advancing and extending the light period by 8 hours, which acutely induced Tshb expression, within one day. In a genome-wide expression analysis under this condition, we searched for candidate upstream genes by looking for expression that preceded Tshb’s, and identified Eya3 gene. These results elucidate the comprehensive transcriptional photoperiodic response in the PT, revealing the complex regulation of Tshb expression and unexpectedly rapid response to light changes in the mammalian photoperiodic system. Mice were separated into 2 groups. One group was maintained under the short-day conditions (light: dark = 8 h:16 h, ZT0 = lights on, ZT8 = lights off, 400 lux) and the other was housed under long-day conditions (light:dark = 16 h:8 h, ZT0 = lights on, ZT16 = lights off, 400 lux) for 2 weeks. The PTs of both groups were retrieved every 4 h for 1 day (6 time points for each group), starting at ZT0. For the experiments performed during the first day of the long-day conditions, we applied two different conditions, following 3 weeks under short-day conditions. In one, the light-onset was advanced by 8 hours (advance condition), and in the other, the dark period was delayed by 8 hours (delay condition). PTs from both groups were obtained every 4 h for 1 day, starting at the lights-on time. (Lights on for the advance condition was ZT16 as defined by the short-day condition. Lights on for the delay condition was ZT0). We sampled 25 mice at each time point. This whole procedure was repeated twice (n = 2) to obtain experimental replicates.

Dataset Information

Transcription profiling of Coturnix japonica during the photoinduction process.

Publications

Thyrotrophin in the pars tuberalis triggers photoperiodic response.

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