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Transcription profiling of human resting and IL2 activated natural killer cells

ABSTRACT: The aim of the study was to investigate the activation of human NK cells by IL2 through analyzing the global gene expression at different time points (0, 2, 8 and 24 hours) after culture with the cytokine IL2 at 100 IU/ml. NK cells with the CD56+/CD16+ and CD3- phenotype were negatively selected by immunomagnetic beads and re-examined by flow-cytometry to ensure greater than 90% purity . Experiment Overall Design: RNA was extracted and pooled from 4 different donors, amplified and labled according to the manufacturer’s instruction (GeneChipU133plus2® , Affymetrix Inc, CA).

ORGANISM(S): Homo sapiens

SUBMITTER: Javeed Iqbal 

PROVIDER: E-GEOD-8059 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome wide transcriptional analysis of resting and IL2 activated human natural killer cells: gene expression signatures indicative of novel molecular signaling pathways.

Dybkaer Karen K   Iqbal Javeed J   Zhou Guimei G   Geng Huimin H   Xiao Li L   Schmitz Alexander A   d'Amore Francesco F   Chan Wing C WC  

BMC genomics 20070710

<h4>Background</h4>Human natural killer (NK) cells are the key contributors of innate immune response and the effector functions of these cells are enhanced by cytokines such as interleukine 2 (IL2). We utilized genome-wide transcriptional profiling to identify gene expression signatures and pathways in resting and IL2 activated NK cell isolated from peripheral blood of healthy donors.<h4>Results</h4>Gene expression profiling of resting NK cells showed high expression of a number of cytotoxic fa  ...[more]

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