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Zfp281 Coordinates Opposite Functions of Tet1 and Tet2 for Alternative Pluripotent States [RNA-Seq]

ABSTRACT: Pluripotent cell identity comprises a spectrum of cell states including naive and primed states, which are typified by mouse embryonic stem cells (ESCs) and epiblast-derived stem cells (EpiSCs), respectively. Here we define a pluripotent cell fate (PCF) gene signature based on RNA-seq analysis associated with naive and primed pluripotency acquisition, and identify Zfp281 as a key transcriptional regulator for primed pluripotency and also as a barrier to achieve the naive pluripotency of both mouse and human ESCs. RNA sequencing analysis was performed in WT and Zfp281 null mouse embryonic stem cells under different pluripotent culture conditions. RNA-seq Experiments were carry out in two biological replciates. Genome binding/occupancy profiling of Zfp281 was performed in mouse embryonic stem cells by ChIP sequencing.

ORGANISM(S): Mus musculus

SUBMITTER: Jianlong Wang

PROVIDER: E-GEOD-81044 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Zfp281 Coordinates Opposing Functions of Tet1 and Tet2 in Pluripotent States.

Fidalgo Miguel M Huang Xin X Guallar Diana D Sanchez-Priego Carlos C Valdes Victor Julian VJ Saunders Arven A Ding Junjun J Wu Wen-Shu WS Clavel Carlos C Wang Jianlong J

Cell stem cell 20160623 3

Pluripotency is increasingly recognized as a spectrum of cell states defined by their growth conditions. Although naive and primed pluripotency states have been characterized molecularly, our understanding of events regulating state acquisition is wanting. Here, we performed comparative RNA sequencing of mouse embryonic stem cells (ESCs) and defined a pluripotent cell fate (PCF) gene signature associated with acquisition of naive and primed pluripotency. We identify Zfp281 as a key transcription ...[more]

PMID: 27345836

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