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Bulk RNA-seq analysis of Type 5 retinal bipolar cells from the Htr3a-GFP line

ABSTRACT: 15,000 GFP+ cells were collected from two replicates of the Htr3a GFP line into RNAlater (ThermoFisher, AM7024). RNA was purified and bulk RNA-seq was performed using the Ovation RNA-seq system V2 (Nugen, 7102-32) Bulk RNA-seq analysis of Type 5 retinal bipolar cells (2 biological replicates)

ORGANISM(S): Mus musculus

SUBMITTER: Karthik Shekhar 

PROVIDER: E-GEOD-81902 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Patterns of gene expression can be used to characterize and classify neuronal types. It is challenging, however, to generate taxonomies that fulfill the essential criteria of being comprehensive, harmonizing with conventional classification schemes, and lacking superfluous subdivisions of genuine types. To address these challenges, we used massively parallel single-cell RNA profiling and optimized computational methods on a heterogeneous class of neurons, mouse retinal bipolar cells (BCs). From  ...[more]

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