Determination of the RNA linked to the paraspeckle in the GH4C1 cell line
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ABSTRACT: The goal of this study was to determine the identity of the RNA linked to the paraspeckles in the rat GH4C1 cell line. GH4C1 cell were fixed with 1% paraformaldehyde in PBS. Then nuclei were purified, lysed and sonicated. RNA pull-down was performed using two antisense DNA biotinylated oligonucleotide probes that target the lncRNA Neat1. Streptavidin-magnetic beads were added to hybridization reaction and complexes were captured by magnets. RNA was isolated using NucleoSpin®RNA XS (Macherey-Nagel).
ORGANISM(S): Rattus norvegicus
SUBMITTER: Anne-Marie François-Bellan
PROVIDER: E-GEOD-81972 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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