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Microarray analysis of the effects of IL-2 signaling on in vitro activated WT CD8 T cells

ABSTRACT: Interleukin 2 (IL-2) promotes proliferation and differentiation of CD8+ T cells in vitro and in vivo. To define gene expression regulated by IL-2, we purified naive CD8+ T cells, activated them for 2 days followed by treatment with recombinant IL-2 or with neutralizing antibody against IL-2 and compared gene expression between the two treatments. Total RNA was extracted using the NucleoSpin RNA XS kit (Macherey-Nagel), amplified using a PicoSL RNA amplification kit (Nugen) and biotinylated with Encore biotin module (Nugen). Labeled RNA was hybridized to Mouse Gene 1.0ST microarrays (Affymetrix) according to the manufacturer’s instruction.

ORGANISM(S): Mus musculus

SUBMITTER: Takeshi Egawa

PROVIDER: E-GEOD-58077 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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