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Expression data from whole lateral ventricle choroid plexus tissue of young (two months old) and aged (eighteen months old) CD1 male mice.

ABSTRACT: We used microarrays to reveal the global expression profiles of young and old whole lateral ventricle choroid plexus tissue. RNA was isolated from whole lateral ventricle choroid plexus tissue followed by RNA amplification and hybridization on Affymetrix microarrays. Each sample contains both lateral ventricle choroid plexi from one male CD1 mouse. A total of six samples (three biological replicates from 2 different ages) were analyzed.

ORGANISM(S): Mus musculus

SUBMITTER: Dogukan Mizrak

PROVIDER: E-GEOD-82308 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from whole lateral ventricle choroid plexus tissue of young (two months old) and aged (eighteen months old) CD1 male mice.

Project description:We used microarrays to reveal the global expression profiles of young and old whole lateral ventricle choroid plexus tissue.

2016-07-20 | GSE82308 | GEO

Spatially heterogeneous choroid plexus transcriptomes encode positional identity and contribute to regional cerebrospinal fluid production

Project description:A sheet of choroid plexus epithelial cells extends into each cerebral ventricle and secretes signaling factors into the cerebrospinal fluid (CSF). To evaluate whether differences in the CSF proteome across ventricles arise, in part, from regional differences in choroid plexus gene expression, we defined the transcriptome of lateral ventricle (telencephalic) vs. fourth ventricle (hindbrain) choroid plexus. We find that positional identities of mouse, macaque, and human choroid plexi derive from gene expression domains that parallel their axial tissues of origin. We then show that molecular heterogeneity between telencephalic and hindbrain choroid plexi contributes to region-specific, age-dependent protein secretion in vitro. Transcriptome analysis of FACS-purified choroid plexus epithelial cells also predicts their cell type-specific secretome. Spatial domains with distinct protein expression profiles were observed within each choroid plexus. We propose that regional differences between choroid plexi contribute to dynamic signaling gradients across the mammalian cerebroventricular system.

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Project description:The choroid plexus (ChP) in each brain ventricle produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier. We apply single cell and single nuclei sequencing to identify region and age specific shifts in gene expression of the constituent cell types of the choroid plexus. First, we sequenced whole cells (15,620) from each ventricle (lateral, third and fourth) of the embryonic mouse brain (Embryonic day (E) 16.5). Our analyses and validation of gene (smFISH) and protein (immunohistochemistry) expression combined with spatial mapping (confocal imaging) revealed the identity and location of major cell types, subtypes, proliferating cells, progenitor populations and regionalized gene expression, across each ventricle in the developing brain. Next, to track age-dependent shifts in the choroid plexus properties, we performed single nuclei sequencing (83,040) across each ventricle of the embryonic (E16.5), adult (4 months) and aging (20 months) mouse brain. Epithelial and mesenchymal cells showed regionalized gene expression patterns by ventricle, starting at embryonic stages and persisting with age. Dramatic transcriptional shifts were found with maturation (embryonic to adult) and a smaller shift within each aged cell type. With aging, epithelial cells upregulated host defense programs and resident macrophages enhanced expression of IL-1b signaling genes. Our atlas revealed ChP brain barrier cellular diversity, architecture and signaling across ventricles during development, maturation and aging.

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Gene expression profiles obtained from laser-microdissected human choroid plexus papilloma cells

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The evolutionarily conserved zebrafish choroid plexus maintains the size of brain ventricles

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Genome-wide analysis and comparison of E15 and adult rat lateral ventricular choroid plexus epithelial cells.

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Choroid Plexus Expression Data from Add1 Null Mice on the C57BL/6J Background Strain

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