Unknown,Transcriptomics,Genomics,Proteomics

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Transcript profiling in S2 cells and salivary glands.


ABSTRACT: Two chromosome-targeting and gene regulatory systems are present in Drosophila melanogaster. The male X-chromosome is targeted by the male-specific lethal complex believed to mediate the two-fold up-regulation of the X-linked genes. The highly heterochromatic 4th chromosome is specifically targeted by the Painting of Fourth (POF) protein which together with heterochromatin protein 1 (HP1) modulate the expression level of genes on the 4th chromosome. Here we report high-resolution mapping of POF and HP1 on the 4th chromosome using chromatin immunoprecipitation followed by tiling microarrays (ChIP-chip) in S2 cells and salivary glands. The enrichments are compared to transcript profiles of the two cell types used. POF specifically binds to genes with a strong preference for exons. The POF binding profile correlates to the binding profile of HP1 which in addition displays a typical M-bM-^@M-^\peakM-bM-^@M-^] in the promoter regions of bound genes. POF and HP1 binds to active genes and the binding correlates with levels of transcription and changes in binding levels are paralleled by a comparable change in transcription. Our results provide a high resolution description of the chromosome 4 specific gene regulatory system provided by the combinatorial effects of POF and HP1. Keywords: Transcript profiling Transcript profiling in S2 cells and salivary glands. Two biological replicates, one replicate per array.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Per Stenberg 

PROVIDER: E-GEOD-8297 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

POF and HP1 bind expressed exons, suggesting a balancing mechanism for gene regulation.

Johansson Anna-Mia AM   Stenberg Per P   Pettersson Fredrik F   Larsson Jan J  

PLoS genetics 20071101 11


Two specific chromosome-targeting and gene regulatory systems are present in Drosophila melanogaster. The male X chromosome is targeted by the male-specific lethal complex believed to mediate the 2-fold up-regulation of the X-linked genes, and the highly heterochromatic fourth chromosome is specifically targeted by the Painting of Fourth (POF) protein, which, together with heterochromatin protein 1 (HP1), modulates the expression level of genes on the fourth chromosome. Here we use chromatin imm  ...[more]

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