Transcription profiling of rat testis cell cultures grown on Sertoli cell and fibroblast feeder cell cultures
Ontology highlight
ABSTRACT:
ORGANISM(S): Rattus norvegicus
SUBMITTER: Nikolaus Schultz
PROVIDER: E-GEOD-830 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Defining the spermatogonial stem cell.
Hamra F Kent FK Schultz Nikolaus N Chapman Karen M KM Grellhesl Dana M DM Cronkhite Jennifer T JT Hammer Robert E RE Garbers David L DL
Developmental biology 20040501 2
Through the use of donor cells from transgenic rats expressing GFP exclusively in the germline, we have defined culture conditions where male germ cells lose (on STO cells) or maintain (on MSC-1 cells) stem cell activity. A cadre of germ cell transcripts strikingly decrease in relative abundance as a function of testis age or culture time on STO cells, but only a subset of these transcripts (approximately 248) remain elevated when cultured on MSC-1 cells. If specific gene expression regulates st ...[more]
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