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Transcription profiling of porcine olfactory bulb neuroblasts and PK15 non-neuronal, epithelial origin cell cultures

ABSTRACT: The phenotypically characterized hTERT immortalized porcine olfactory bulb neuroblast cell line (OBGF400) was subjected to an extensive whole genome-scaled expression profile for establishing their use as an in vitro neuronal disease model system. Microarrays were used to provide a comprehensive knowledge underlying the genomic complexity and overall gene expression capacity of the immortalized OBGF400 cells. The analysis revealed the elaborate signaling mechanisms of this unique subpopulation of porcine neuronally committed progenitors that mirrors the intricate organization of postnatal neurongenic zones. SUBMITTER_CITATION: Transcriptome Profile and Cytogenetic Analysis of Immortalized Neuronally Restricted Progenitor Cells Derived from the Porcine Olfactory Bulb. Animal biotechnology 2009 vol:20 iss:4 page:186-215 Experiment Overall Design: Total cellular RNA extracts from independent OBGF400 (neuroblasts) and PK15 (non-neuronal, epithelial origin) cell cultures (9 each) were pooled into a total of 3 biological replicates per cell line. The concentrations and purity of the pooled RNA preparations were determined using the BioAgilent RNA assay prior to hybridization on Affymetrix GeneChipÂ® Porcine Genome Expression Arrays. To ascertain the genes that were preferentially expressed by the OBGF400 neuroblasts, we used the PK15 cellular array in an effort to exclude somatic cell background.

ORGANISM(S): Sus scrofa

SUBMITTER: Gail Scherba

PROVIDER: E-GEOD-16855 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from OBGF400 cell line (porcine olfactory bulb neuroblast origin)

Project description:The phenotypically characterized hTERT immortalized porcine olfactory bulb neuroblast cell line (OBGF400) was subjected to an extensive whole genome-scaled expression profile for establishing their use as an in vitro neuronal disease model system. Microarrays were used to provide a comprehensive knowledge underlying the genomic complexity and overall gene expression capacity of the immortalized OBGF400 cells. The analysis revealed the elaborate signaling mechanisms of this unique subpopulation of porcine neuronally committed progenitors that mirrors the intricate organization of postnatal neurongenic zones.

2009-10-30 | GSE16855 | GEO

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Project description:Porcine Olfactory Bulb RNA-Sequencing

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Project description:The generation of distinct cell fates during development depends on asymmetric cell division of progenitor cells. In the central and peripheral nervous system of Drosophila, progenitor cells respectively called neuroblasts or sensory organ precursors use PAR polarity during mitosis to control cell fate determination in their daughter cells. How polarity and the cell cycle are coupled, and how the cell cycle machinery regulates PAR protein function and cell fate determination is poorly understood. Here, we generate an analog sensitive allele of CDK1 and reveal that its partial inhibition weakens but does not abolish apical polarity in embryonic and larval neuroblasts and leads to defects in polarisation of fate determinants. We describe a novel in vivo phosphorylation of Bazooka, the Drosophila homolog of PAR-3, on Serine180, a consensus CDK phosphorylation site. In some tissular contexts, phosphorylation of Serine180 occurs in asymmetrically dividing cells but not in their symmetrically dividing neighbours. In neuroblasts, Serine180 phosphomutants disrupt the timing of basal polarisation. Serine180 phosphomutants also affect the specification and binary cell fate determination of sensory organ precursors as well as Baz localisation during their asymmetric cell divisions. Finally, we show that CDK1 phosphorylates Serine-S180 and an equivalent Serine on human PAR-3 in vitro.

2024-07-02 | PXD051560 | Pride

Single cell RNA sequencing of interneurons of the mouse dorsolateral striatum II

Project description:In order to investigate age-dependent mRNA expression in mouse striatal interneurons, we performed single cell RNA-seq on FACS-isolated fluorescently labeled cells from the dorsal striatum of two mouse lines, 5ht3aEGFP or Lhx6cre::R26R-tdTomat, from either P21-26 or P55-76 animals. We included the ventricular side of the striatum that contains adult born neuroblasts destined for the olfactory bulb.

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Activation of a neural stem cell transcriptional program in parenchymal astrocytes

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2020-08-01 | E-MTAB-9268 | biostudies-arrayexpress

Transcription profiling of human normal neuroblasts vs. malignant neuroblastomas (low- and high-stage)

Project description:Comparison of normal neuroblasts with malignant neuroblastomas (low- and high-stage)

2006-12-18 | E-MEXP-669 | biostudies-arrayexpress

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Project description:The goal of this study is profile gene expression patterns of Foxr2-induced brain tumor cells. Foxr2 overexpression in the brain induced tumor formation in the olfactory bulb (OB) and the brainstem (BS) under the p53 deficient background. Tumor cells were cultured in vitro, which resulted in sphere formation. Sphere-forming cells were used for gene expression analysis. Immortalized primary astroglia (AG) was analzyed for comparison.

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Ecdysone and Mediator trigger a metabolic switch uncoupling cell cycle from cell growth to end proliferation in Drosophila neural stem cells

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