Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Generation of new compounds through unbalanced transcription of landomycin A cluster


ABSTRACT: On the example of the biosynthetically exhausted landomycin A cluster we demonstrate unbalancing of gene transcription as an efficient method for the generation of new compounds. Decoupled from the native regulators LanI and LanK, all landomycin A structural genes were set under the control of a single synthetic promoter and expressed in a heterologous host Streptomyces albus J1074. Previously being both temporarily and quantitatively regulated, these genes were transcribed as a single polycistronic mRNA leading to the production of four novel and two known compounds. No glycosylated landomycins were detected though the entire biosynthetic cluster was transcribed, showing the crucial role of the balanced gene expression for the production of landomycin A. Two new compounds, fridamycin F and G, isolated in this study were shown to originate from the interplay between the expressed biosynthetic pathway and metabolic network of the heterologous host. Structure activity studies of the isolated compounds as well as results of transcriptome sequencing are discussed in this article. Comparison of gene expression of the H2-26 cosmid (encoding landomycin A biosynthetic genes) with H2-26-act, where an additional constitutive promoter cassette was integrated to drive biosynthetic genes transcription.

ORGANISM(S): Streptomyces albus

SUBMITTER: Bogdan Tokovenko 

PROVIDER: E-GEOD-83137 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2016-07-01 | GSE83137 | GEO
2015-08-06 | E-GEOD-71738 | biostudies-arrayexpress
2014-10-17 | E-GEOD-62434 | biostudies-arrayexpress
2013-04-24 | E-GEOD-41145 | biostudies-arrayexpress
2024-02-26 | PXD040286 | Pride
2022-01-10 | PXD030484 | Pride
2016-02-22 | E-GEOD-66194 | biostudies-arrayexpress
2016-09-19 | E-MTAB-5062 | biostudies-arrayexpress
2017-06-01 | E-MTAB-3806 | biostudies-arrayexpress
2017-09-20 | PXD007267 | Pride