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Elucidation of Transcriptome-Wide MicroRNA Binding Sites in Human Cardiac Tissues by Ago2 HITS-CLIP

ABSTRACT: We deciphered Ago2:RNA interactions in post-mortem human heart tissues using crosslinking immunoprecipitation coupled with high-throughput sequencing (HITS-CLIP) to generate the first transcriptome-wide map of miR targeting events in human myocardium, detecting 4000 cardiac Ago2 binding sites across >2200 target transcripts. Profiling AGO2-associated RNAs in cardiac tissues obtained from 6 donors with heart diseases of differing etiologies

ORGANISM(S): Homo sapiens

SUBMITTER: Ryan Boudreau

PROVIDER: E-GEOD-83410 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Elucidation of transcriptome-wide microRNA binding sites in human cardiac tissues by Ago2 HITS-CLIP.

Spengler Ryan M RM Zhang Xiaoming X Cheng Congsheng C McLendon Jared M JM Skeie Jessica M JM Johnson Frances L FL Davidson Beverly L BL Boudreau Ryan L RL

Nucleic acids research 20160714 15

MicroRNAs (miRs) have emerged as key biological effectors in human health and disease. These small noncoding RNAs are incorporated into Argonaute (Ago) proteins, where they direct post-transcriptional gene silencing via base-pairing with target transcripts. Although miRs have become intriguing biological entities and attractive therapeutic targets, the translational impacts of miR research remain limited by a paucity of empirical miR targeting data, particularly in human primary tissues. Here, t ...[more]

PMID: 27418678

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