Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

43 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of the molecular targets of Orm1 in regenerating mouse liver

ABSTRACT: To identify the molecular targets of orosomucoid (Orm1) during liver regeneration, GeneChip analysis was performed at 48 h after partial hepatectomy (PH) in regenerating mouse liver treated with siControl or siOrm. A total of 180 differentially expressed genes in Orm1 konckdown mouse liver by comparing with siControl were identified with a fold change more than 2. Then, pathway analysis performed on the altered gene expression profiles using Ingenuity Pathways Analysis (IPA) program revealed that cell cycle, Toll-like receptor and TGF-beta receptor signaling pathways were under control of Orm1 in regenerating mouse livers. Three days post In vivo knockdown of Orm1 with its siRNA administered to mice using Invivofectamine 3.0 by a single injection, 40% PH was perfomred and gene expression prolifes of regenerating mouse livers at 48 h after PH was measued using Affymetrix GeneChip Mouse Genome 430A 2.0 Array.

ORGANISM(S): Mus musculus

SUBMITTER: Xian-Yang Qin

PROVIDER: E-GEOD-83733 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Identification of the molecular targets of Orm1 in regenerating mouse liver

Project description:To identify the molecular targets of orosomucoid (Orm1) during liver regeneration, GeneChip analysis was performed at 48 h after partial hepatectomy (PH) in regenerating mouse liver treated with siControl or siOrm. A total of 180 differentially expressed genes in Orm1 konckdown mouse liver by comparing with siControl were identified with a fold change more than 2. Then, pathway analysis performed on the altered gene expression profiles using Ingenuity Pathways Analysis (IPA) program revealed that cell cycle, Toll-like receptor and TGF-beta receptor signaling pathways were under control of Orm1 in regenerating mouse livers.

2016-07-01 | GSE83733 | GEO

Mus musculus

Project description:Identification of the molecular targets of Orm1 in regenerating mouse liver

| PRJNA327314 | ENA

Genome wide analysis of liver histone H3K9 acetylation after partial hepatectomy in glucose-treated and control mice.

Project description:We report the results of AcH3K9 ChIP-Seq on regenerating liver harvested 12 h after partial hepatectomy (PH) from mice treated with supplemental parenteral dextrose (to delay onset of PH-induced hypoglycemia) or vehicle (as control). Examination of the effect of delaying PH-induced hypoglycemia on histone H3K9 acetylation in regenerating liver.

2016-02-09 | E-GEOD-71496 | biostudies-arrayexpress

microRNA expression profiling of the normal and regenerating liver of Chiloscyllium plagiosum

Project description:This study aimed to improve our understanding of the mechanisms of liver regeneration in sharks and to identify the microRNAs that participate in liver regeneration and other liver-related diseases. To this end, normal and regenerating liver tissues from C. plagiosum were harvested 0, 3, 6, 12 and 24 h after partial hepatectomy (PH) and were sequenced using the Illumina/Solexa platform. In total, 309 known microRNAs and 590 novel microRNAs were identified in C. plagiosum. There were 368 microRNAs differentially expressed between the normal and regenerating livers. Using target prediction and GO analysis, most of the differentially expressed microRNAs were assigned to functional categories that may be involved in regulating liver regeneration, such as cell proliferation, differentiation and apoptosis. Additionally, this study adds several novel microRNAs to the database, which will help identify microRNAs in other genetically related species and provides a starting point for future studies aimed at understanding the roles of microRNAs in liver regeneration and other liver diseases.

2016-08-30 | GSE72339 | GEO

Genome wide analysis of liver histone H3K9 acetylation after partial hepatectomy in glucose-treated and control mice.

2016-02-09 | GSE71496 | GEO

Single nuclei sequencing in wild-type and Cox10-/- regenerating liver.

Project description:We report single nuclei sequencing in wild-type and Cox10-/- regenerating mouse livers.

2023-07-02 | GSE236043 | GEO

Loss of claudin-3 impairs hepatic metabolism, biliary-barrier function and cell proliferation in the murine liver

Project description:We determined the functional role of the tight junction protein Claudin-3 for overall gene expression in the liver. In the bulk RNAseq, we compare global gene expression in the livers of C57BL/6 wildtype mice and in CLDN3-/- mice. We sequenced both normal liver tissue and regenerating liver tissue.

2021-07-01 | GSE159914 | GEO

H3K27ac ChIP-seq in wild-type and Cox10-/- regenerating liver.

Project description:We report here the genome-wide deposition of H3K27ac marks in wild-type and Cox10-/- regenerating mouse livers.

2023-07-01 | GSE235879 | GEO

Dynamic remodeling of translation programs drives hepatocyte proliferation during liver regeneration

Project description:During liver regeneration, most new hepatocytes arise from pre-existing ones; yet, the underlying mechanisms that drive these cells from quiescence to proliferation remain poorly defined. By using high-resolution transcriptome profiling of polysome fractions from purified hepatocytes isolated from quiescent and toxin-injured adult mouse livers,we uncover the mRNA transcripts regulated during liver regeneration. The translational remodeling modulates protein levels of a set of splicing factors including Epithelial splicing regulatory protein 2 (ESRP2), which activates an early postnatal splicing program in regenerating hepatocytes as well as metabolic genes.

2018-09-17 | GSE106140 | GEO

XBP1 coregulates unfolded protein response, acute phase response and DNA damage response during mouse liver regeneration

Project description:Partial hepatectomy (PH) imposes increased protein synthesis demands on remaining hepatocytes. Activation of IRE1α, a key sensor of endoplasmic reticulum (ER) stress, elicits XBP1 mRNA splicing and production of XBP1 protein, a main trigger of the unfolded protein response (UPR). Using genome-wide ChIPseq analysis we have explored the role of XBP1 during liver regeneration. XBP1 was induced in liver at 6h after PH in an IL-6 dependent manner. After PH, XBP1 silencing caused persistent ER stress, defective acute phase response (APR), increased hepatocellular damage and regenerative delay. At 6h post-PH, XBP1 bound an increased number of genes implicated in proteostasis, APR, metabolism, antioxidant defense and DNA damage response (DDR). ). XBP1 was linked to regulatory sequences containing canonical UPR motifs as well as motifs characteristic of other nuclear factors suggesting molecular interactions during liver regeneration. Upon PH, XBP1 bound the promoter of STAT3, a molecule downregulated in XBP1-silenced livers. XBP1 was indispensable for ser727-STAT3 phosphorylation, a post-translational modification implicated in cell proliferation and DNA damage repair. During active DNA replication XBP1 deficient livers showed high levels of the DNA double-strand break marker γ-H2AX, in association with defective upregulation of Eme1 and Fen1, two main executors of DDR. In conclusion, XBP1 is induced after PH and co-regulates UPR, APR and DDR during liver regeneration.

2017-01-17 | GSE86048 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data