Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Effect of JAK blockade on IFNa response in B cells in vitro


ABSTRACT: B cells respond robustly to type 1 interferons which signal through JAK1 and TYK2. Here we analyzed the effects of a panel of JAK inhibitors on the IFNa transcriptional response in activated B cells in vitro. Splenic B cells were isolated by negative selection from 6wk old male C57BL/6 mice. These were activated with LPS(10ug/ml) for 48hrs and thereafter cultured with IFNa (100U/ml) in the presence or absence of JAK inhibitors at IC80 for 2hrs or 8hrs in vitro. Cells were then transferred directly into TriZol. RNA was prepared in Trizol for gene expression profiling by Affymetrix Mouse Gene 1.0 ST Arrays.

ORGANISM(S): Mus musculus

SUBMITTER: CBDM Lab 

PROVIDER: E-GEOD-84560 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2016-07-20 | E-GEOD-84565 | biostudies-arrayexpress
2016-07-20 | E-GEOD-84561 | biostudies-arrayexpress
2016-07-20 | E-GEOD-84566 | biostudies-arrayexpress
2016-07-20 | GSE84560 | GEO
2016-07-20 | GSE84565 | GEO
2015-11-20 | E-GEOD-75194 | biostudies-arrayexpress
2016-07-20 | E-GEOD-84562 | biostudies-arrayexpress
2016-07-27 | E-GEOD-84853 | biostudies-arrayexpress
2015-11-20 | E-GEOD-75195 | biostudies-arrayexpress
2016-07-20 | E-GEOD-84564 | biostudies-arrayexpress