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Effects of Androstenedione, Ethinyl Estradiol, and Prednisone on Gene Expression in the Male Mouse Liver

ABSTRACT: The goal of this study was to provide insights into the adverse outcome pathways that underlie the liver tumor effects of androstenedione in the mouse liver by comparing gene expression patterns to two liver noncarcinogens, ethinyl estradiol and prednisone. B6C3F1 male mice were exposed for 7 days to control diet or diet containing ethinyl estradiol (EE2) at 1, 2.5, 5, or 10 ppm or prednisone (PRED) at 1.25, 2.5, 5, 25 ppm. Androstenedione (A4) was administered in 0.5% carboxymethyl cellulose by gavage at 2, 10, 20, and 50 mg/kg/day. Separate groups of mice were exposed to 50 mg/kg/day A4, 10 ppm EE2 or 25 ppm PRED for 28 days.

ORGANISM(S): Mus musculus

SUBMITTER: J Corton

PROVIDER: E-GEOD-84590 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Effects of Androstenedione, Ethinyl Estradiol, and Prednisone on Gene Expression in the Male Mouse Liver

Project description:The goal of this study was to provide insights into the adverse outcome pathways that underlie the liver tumor effects of androstenedione in the mouse liver by comparing gene expression patterns to two liver noncarcinogens, ethinyl estradiol and prednisone.

2016-07-28 | GSE84590 | GEO

Mus musculus

Project description:Effects of Androstenedione, Ethinyl Estradiol, and Prednisone on Gene Expression in the Male Mouse Liver

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Transcription profiling of liver from sticklebacks exposed to copper, dibezanthracene or ethinyl-estradiol

Project description:Stickleback were exposed to Cu, DbA (Dibezanthracene), EE2 (Ethinyl-estradiol) or relevant controls. Liver tissue from individual fish exposed to different doses were arrayed versus a common reference.

2009-07-10 | E-MAXD-42 | biostudies-arrayexpress

Transcription profiling of Stickleback liver exposed to Cu, DbA (Dibezanthracene), EE2 (Ethinyl-estradiol) or relevant controls

Project description:Stickleback were exposed to Cu, DbA (Dibezanthracene), EE2 (Ethinyl-estradiol) or relevant controls. Pooled liver tissue from treatment groups exposed to different doses were arrayed versus a common reference.

2009-07-10 | E-MAXD-43 | biostudies-arrayexpress

Alteration in liver proteome caused by ethinyl estradiol in males Cyprinodon variegatus

Project description:Adult male marine fish were exposed to 100ng/L of ethinyl estradiol (EE2) for 7 days in 1.8L vessels, dimethyl sulfoxide (DMSO) was used as carrier never reaching concentrations > 0.001% v/v. The same volume of DMSO was added to the solvent control vessels. Culture conditions, monitored daily, were as follows: temperature 18.8 ± 0.2°C, pH 7.94±0.03, dissolved oxygen saturation 99.3% ± 1.1 and 16:8 hours of light:dark photoperiod. 90% of the water was renewed daily and EE2 and DMSO concentrations were adjusted after that.

2022-05-23 | PXD027547 | Pride

Transcriptomic responses of three-spined stickleback (Gasterosteus aculeatus) from Aire and Siblyback populations to ethinyl-estradiol, copper and binary mixtures

Project description:Sticklebacks were caught from the River Aire (Yorkshire, UK) and Siblyback reservoir (Cornwall, UK), depurated for 4 months at Exeter University, split into groups of 20 individuals before exposure. Fish were exposed to either control conditions, three concentrations of copper (3.2, 32, 128 Âµg/L; prepared using copper sulphate), three concentrations of ethinyl-estradiol (EE2) (1, 10, 32 ng/L) and three mixtures (3.2 Âµg Cu/L and 32 ng EE2/L; 128 Âµg Cu/L and 1 ng EE2/L; 128 Âµg Cu/L and 32 ng EE2/L) in duplicate tanks for 4 days. After sampling liver of male fish were stored at -80C for transcriptomics. Total RNA was prepared from fish livers, aliquots of all samples were pooled and labeled with Cy3-dCTP as a common reference, individual samples were labeled with Cy5-dCTP. Each hybridisation to the stickleback PGPS2 cDNA microarray consisited of one Cy5-labeled individual sample and one aliquot of the Cy3 labeled commmon reference sample. Data were captured with an Axon scanner using Genepix software.

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High-fat or ethinyl-estradiol intake during pregnancy increases mammary cancer risk in several generations of offspring

Project description:Maternal exposures during pregnancy influence the risk of many chronic adult-onset diseases in the offspring. We investigated whether feeding pregnant rats a high fat (HF) or ethinyl-estradiol (EE2)-supplemented diet affects carcinogen-induced mammary cancer risk in daughters, granddaughters and great-granddaughters. Here we show that mammary tumorigenesis is higher in daughters and granddaughters of HF rat dams and in daughters, granddaughters and great-granddaughters of EE2 rat dams. Outcross experiments indicate that increased mammary cancer risk is transmitted to HF granddaughters equally through the female or male germlines, but it is only transmitted to EE2 granddaughters through the female germline. The effects of maternal EE2 exposure on offspring's mammary cancer risk are associated with alternations in the DNA methylation machinery and methylation patterns in mammary tissue of all three EE2 generations. We conclude that dietary and estrogenic exposures in pregnancy increase breast cancer risk in multiple generations of offspring, possibly through non-genetic means

2012-09-20 | GSE40251 | GEO

High-fat or ethinyl-estradiol intake during pregnancy increases mammary cancer risk in several generations of offspring

2012-09-20 | E-GEOD-40251 | biostudies-arrayexpress

Anchoring ethinylestradiol induced gene expression changes with testicular morphology and reproductive function in the medaka

Project description:This study assessed the implications of a 14 day sub-chronic exposure of ethinylestradiol (EE2; 1.0 or 10.0 M-BM-5g/L EE2) on male medaka fertility, testicular histology and testicular gene expression. The findings demonstrate that a 14 day exposure to EE2 induced impaired male reproductive capacity and time- and dose-dependent alterations in testicular morphology and gene expression. The average fertilization rate/day following the exposure for control, 1.0 and 10.0 M-BM-5g/L EE2 was 91.3% (M-BM-14.4), 62.8% (M-BM-18.3) and 28.8% (M-BM-15.8), respectively. The testicular morphologic alterations observed include increased germ cell apoptosis, decreased germinal epithelium and thickening of the interstitium. The morphologic changes observed were highly associated with gene expression changes observed using a medaka-specific microarray. A pathway analysis of the differentially expressed genes emphasized genes and pathways associated with apoptosis, cell cycle and proliferation, collagen production/extracellular matrix organization, hormone signaling, male reproduction and protein ubiquitination among others. Six month old male medaka were exposed to ethinyl estradiol (EE2) for a 14 day time period. Treatment exposures were completed in triplicate including DMSO (vehicle control), 1.0 M-BM-5g/L EE2, and 10.0 M-BM-5g/L EE2. Fish were sampled for gene expression on days 1, 7 and 14 of exposure. Five male fish were placed in 2-liter beaker replicates for each treatment and sampling time point.

2013-03-06 | E-GEOD-44859 | biostudies-arrayexpress

RNA Expression Data from Bovine Ovarian Granulosa Cells from High or Low Androgen-Content Follicles

Project description:Excessive androgen production is a hallmark of human and bovine ovarian disorders, and our lab has identified a naturally-occurring bovine model with excess follicular fluid androstenedione (A4) without a proportional estrogen increase. Granulosa cell populations from both the control, Low A4 follicles and the abnormal, High A4 follicles were isolated. Total RNA was extracted from those granulosa cells and submitted to the University of Nebraska Medical Center Microarray Core Facility for transcriptome quantification using Affymetrix Bovine GeneChip Gene 1.0 ST Arrays. The orignal Affymetrix .CEL files and the normalized linear expression data are included in this submission.

2019-04-30 | GSE97017 | GEO

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