Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Hsd:ICR(CD-1) mouse intestines to study regional variations in ABC transporter expression along the mouse intestinal tract.


ABSTRACT: 8 week-old male Hsd:ICR(CD-1) mice were fed ad libitum a standard chow (Harlan Teklad diet 2018S) and housed in groups of five. Animals were then divided into 3 pools (n=10) and sacrificed. The small intestine was extracted and divided into three sections, where the first 2-3 cm after the stomach comprised the duodenum, the middle third the jejunum, and the section before the ileo-ceco-colic junction comprised the ileum. Colon was not divided into proximal and distal sections and was treated as a single intestinal section.

ORGANISM(S): Mus musculus

SUBMITTER: Pascale Anderle 

PROVIDER: E-GEOD-849 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Regional variations in ABC transporter expression along the mouse intestinal tract.

Mutch David M DM   Anderle Pascale P   Fiaux Muriel M   Mansourian Robert R   Vidal Karine K   Wahli Walter W   Williamson Gary G   Roberts Matthew-Alan MA  

Physiological genomics 20040312 1


The ATP-binding cassette (ABC) family of proteins comprise a group of membrane transporters involved in the transport of a wide variety of compounds, such as xenobiotics, vitamins, lipids, amino acids, and carbohydrates. Determining their regional expression patterns along the intestinal tract will further characterize their transport functions in the gut. The mRNA expression levels of murine ABC transporters in the duodenum, jejunum, ileum, and colon were examined using the Affymetrix MuU74v2 G  ...[more]

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