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Global Transcriptional Analysis of Mycoplasma hyopneumoniae Following Exposure to Norepinephrine


ABSTRACT: The goal of this study is to gain a better understanding of the transcriptional responses of M. hyopneumoniae under norepinephrine stress. Six independent RNA samples from norepinephrine-exposed cultures were paired with six independent RNA samples from control cultures for hybridization to six two-color microarrays. For three arrays, the control RNA sample was labeled with Cy3 dye and the experimental RNA sample was labeled with Cy5 dye, the dyes were reversed for the other three arrays to overcome dye bias. Keywords: stress response Six independent RNA samples from norepinephrine-exposed cultures were paired with six independent RNA samples from control cultures for hybridization to six two-color microarrays. For three arrays, the control RNA sample was labeled with Cy3 dye and the experimental RNA sample was labeled with Cy5 dye, the dyes were reversed for the other three arrays to overcome dye bias.

ORGANISM(S): Mycoplasma hyopneumoniae

SUBMITTER: Chris Minion 

PROVIDER: E-GEOD-8494 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global transcriptional analysis of Mycoplasma hyopneumoniae following exposure to norepinephrine.

Oneal Michael J MJ   Schafer Erin R ER   Madsen Melissa L ML   Minion F Chris FC  

Microbiology (Reading, England) 20080901 Pt 9


Mycoplasma hyopneumoniae, a component of the porcine respiratory disease complex, colonizes the respiratory tract of swine by binding to the cilia of the bronchial epithelial cells. Mechanisms of pathogenesis are poorly understood for M. hyopneumoniae, but previous work has indicated that it responds to the environmental stressors heat shock, iron deprivation and oxidative compounds. For successful infection, M. hyopneumoniae must effectively resist host responses to the colonization of the resp  ...[more]

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