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DNA methylation patterns of lupus patient PBMCs, subdivided based on protein expression of ARID3a

ABSTRACT: Methyl-seq data was obtained from total peripheral blood mononuclear cells of two patients with systemic lupus erythematosus who were characterized as having>2 standard deviations more ARID3a-expressing B lymphocytes than healthy controls. Similarly, methyl-seq data was also obtained from two SLE patient samples with normal (low) numbers of ARID3-expressing B lymphocytes. Our previous studies showed that increased ARID3a expression in B lymphocytes was associated increased disease activity. Data were generated on an Illumina Hiseq 2000 with paired-end 100bp reads and quality control metrics were assessed with Picard tools. Methylation profiles of genomic DNA from four SLE patient PBMC samples, two with high numbers of ARID3a expressing B cells (ARID3aH) versus two with normal numbers of ARID3a+ B cells (ARID3aN), were generated on an Illumina Hiseq 2000 with paired-end 100bp reads.

ORGANISM(S): Homo sapiens

SUBMITTER: Carol Webb

PROVIDER: E-GEOD-84965 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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DNA methylation patterns of lupus patient PBMCs, subdivided based on protein expression of ARID3a

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