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Single cell RNA-seq of basal, luminal and total epithelial cells from the mammary glands of female mice

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Mammary glands were collected from female mice of various ages: pre puberty (2 weeks), pubescent (5 weeks), adult (10 weeks) and pregnant (12.5 days mid-pregnancy). Freshly sorted epithelial cells were submitted to a Fluidigm C1 System machine for single cell capture and cDNA synthesis. In some cases, cells were further sorted into basal and luminal subsets. Cells were visualized under a microscope to ensure integrity of the captured single cells prior to cDNA preparation. Libraries were sequencing on an Illumina HiSeq 2000 to achieve 100bp paired-end reads or on an Illumina NextSeq to achieve 75bp paired-end reads.

ORGANISM(S): Mus musculus

PROVIDER: GSE98131 | GEO | 2017/11/20

SECONDARY ACCESSION(S): PRJNA384039

REPOSITORIES: GEO

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