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Transcription profiling of human H295R adrenocortical carcinoma cells to investigate OH-PBDE-induced gene expression

ABSTRACT: Affymetrix high-density oligonucleotide microarray analysis was performed to analyse hydroxylated polybrominated diphenyl ethers (OH-PBDE)-induced gene expression changes in H295R adrenocortical carcinoma cells. Cells were treated with growth medium in the presence or absence of 10 µM 2-OH-BDE47 or 2-OH-BDE85 for 24 hours before the gene expression changes were investigated. Experiment Overall Design: Gene expression changes in response to hydroxylated polybrominated diphenyl ethers (OH-PBDEs) were analysed by Microarray technology in H295R adrenocortical carcinoma cells. Cells were treated with 10 µM of 2-OH-BDE47 or 2-OH-BDE85 (or growth medium alone) for 24 hours before the OH-PBDE-induced gene expression changes were investigated. Control, 2-OH-BDE47- and 2-OH-BDE85-treated samples were collected from three independent experiments each.

ORGANISM(S): Homo sapiens

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PROVIDER: E-GEOD-8588 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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OH-PBDE-induced gene expression profiling in H295R adrenocortical carcinoma cells

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