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Gene expression after transfection of IGF-II specific siRNAs

ABSTRACT: Total RNA was isolated from HuH-7 cells after transfection of IGF-II specific siRNAs. Gene expression profiling was performed using the Affymetrix Human Genome U133A 2.0 Arrays. The raw data were analysed using mixed model ANOVA. Keywords: transfection with siRNA Cells transfected with two independent siRNAs (ds1 and ds2) as well as untransfected cells were examined. For each condition biological duplicates were used for gene expression profiling

ORGANISM(S): Homo sapiens

SUBMITTER: Xiaolei Yu

PROVIDER: E-GEOD-8714 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Autocrine insulin-like growth factor-II stimulation of tumor cell migration is a progression step in human hepatocarcinogenesis.

Nussbaum Tanja T Samarin Jana J Ehemann Volker V Bissinger Michaela M Ryschich Eduard E Khamidjanov Akmal A Yu Xiaolei X Gretz Norbert N Schirmacher Peter P Breuhahn Kai K

Hepatology (Baltimore, Md.) 20080701 1

<h4>Unlabelled</h4>The protumorigenic insulin-like growth factor (IGF)-II is highly expressed in a significant fraction of human hepatocellular carcinomas (HCC). However, a functional dissection that clarifies the contribution of IGF-II-binding receptors in tumor progression and a respective molecular characterization of IGF-II signaling has not been performed. Therefore, expression of IGF-II and its receptors IGF-receptor type I (IGF-IR) and insulin receptor (IR) was efficiently blocked using s ...[more]

PMID: 18537183

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Gene expression after transfection of IGF-II specific siRNAs

Project description:Total RNA was isolated from HuH-7 cells after transfection of IGF-II specific siRNAs. Gene expression profiling was performed using the Affymetrix Human Genome U133A 2.0 Arrays. The raw data were analysed using mixed model ANOVA. Keywords: transfection with siRNA

2008-08-06 | GSE8714 | GEO

Transfection with Glucuronidase A affects general proteome pattern in Giardia lamblia trophozoites

Project description:Giardia lamblia is an important causative agent of persistent diarrhea in humans, domestic animals, and cattle. Basic research is usually performed with the strain WBC6 and includes genetic manipulations such as transfections. Here, we investigate how transfection with a plasmid causing stable expression of a foreign gene affects the whole proteome pattern. Using shotgun mass spectrometry, we compare the proteomes of untransfected trophozoites to trophozoites transfected with Escherichia coli glucuronidase A (GusA). Besides GusA, which is detected in the transfected trophozoites only, the proteomes of untransfected and transfected trophozoites differ by 132 differentially expressed proteins. In particular, transfection induces antigenic variation. Since transfection causing stable expression affects the proteome pattern, transfection experiments should take into account this effect. Due to a unique peptide panel, GusA is an example for a suitable internal standard for experiments involving transfected cells.

2021-01-07 | PXD022565 | Pride

Human hepatocarcinoma cells : Huh-7 vs. SNU-739

Project description:Transcriptional profiling of human hepatocarcinoma comparing Huh-7 and SNU-739. Two-condition experiment, normalized ratio represented by Huh-7/SNU-739. Biological replicates: 2 Huh-7 replicates, 2 SNU-739 replicates.

2014-04-29 | E-GEOD-57106 | biostudies-arrayexpress

mRNA expression profile modified by microRNA mir-517a (MIR517A) in human hepatocellular carcinoma cell line

Project description:mRNA expression profile modified by stable transfection of microRNA mir-517a (MIR517A) in a human hepatocellular carcinoma cell line Huh-7 Keywords: Hepatocellular carcinoma, Expression array, microRNA microRNA mir-517a (MIR517A) was transfected to Huh-7 cells using GFP-expressing lentiviral vector. Infected cells were selected using flow cytometry and subjected to mRNA expression microarray experiment. The profiles were compared to controls cells infected with only GFP protein.

2011-02-17 | E-GEOD-20679 | biostudies-arrayexpress

A Daphnane Diterpenoid Isolated from Wikstroemia polyantha induces an inflammatory response and modulates miRNA activity

Project description:MicroRNAs (miRNAs) are endogenously expressed single-stranded ~21M-bM-^@M-^S23 nucleotide RNAs that inhibit gene expression post-transcriptionally by binding imperfectly to elements usually within the 3M-bM-^@M-2untranslated region (3M-bM-^@M-2UTR) of mRNAs. Small interfering RNAs (siRNAs) mediate site-specific cleavage by binding with perfect complementarity to RNA. Here, a cell-based miRNA reporter system was developed to screen for compounds from marine and plant extracts that inhibit miRNA or siRNA activity. The daphnane diterpenoid genkwanine M (GENK) isolated from the plant Wikstroemia polyantha induces an early inflammatory response and can moderately inhibit miR-122 activity in the liver Huh-7 cell line. GENK does not alter miR-122 levels nor does it directly inhibit siRNA activity in an in vitro cleavage assay. Finally, we demonstrate that GENK can inhibit HCV infection in Huh-7 cells. In summary, the development of the cell-based miRNA sensor system should prove useful in identifying compounds that affect miRNA/siRNA activity. Control (no drug treatment): 1 hour and 4 hour samples, 3 replicates each; Drug-treated: 1 hour and 4 hours samples, 3 replicates each

2012-06-28 | E-GEOD-39002 | biostudies-arrayexpress

Transcription profiling of human HeLa cells treated with various functional siRNAs to investigate RNAi off-targets

Project description:Human HeLa cells were treated with various functional siRNAs of different sequence targeting several genes (100 nM or 50 nM for 24 hours). There are two transfection groups - in the first, containing biological duplicates (A and B), the mock-transfected control samples are pooled as a Cy3 reference. The second group is a from a single transfection with each siRNA delivered at two concentrations (50 and 100 nM). A mock-to-untransfected and/or a mock-to-mock self array are control arrays for each group. These sample groups are designated as biological or functional (dose of 100 or 50 nM) duplicates, respectively, in the publication.

2006-03-01 | E-MEXP-668 | biostudies-arrayexpress

Transcription profiling of human HeLa cells in response to IKAP/hELP1 siRNA transfection and/or tumor necrosis factor-a treatment

Project description:HeLa cells were un-transfected or transfected with specific siRNAs (20µM) targeting IKAP/hELP1 or non-specific siRNAs against GFP. Same experiment was done by stimulating the cells for 1 hour with TNF-a (200U/ml). conditions: 1/untransfected 2/siRNAs IKAP 3/untransfected + TNF-a 200U/ml 1h 4/siRNAs IKAP + TNF-a 200U/ml 1h 5/siRNAs GFP + TNF-a 200U/ml 1h

2022-11-21 | E-MEXP-641 | biostudies-arrayexpress

Temporal Endogenous Gene Expression Profiles in Response to Polymer-Mediated Transfection and Profile Comparison to Lipid-Mediated Transfection

Project description:The current understanding of the molecular factors underlying polyethylenimine(PEI)-mediated transfection is largely unknown. Cationic PEI was used to transfer GFP transgene to HEK293T cells. FACS separation of transfected (GFP positive), untransfected (GFP negative), and untreated cells enabled gene expression profiles to be obtained using Affymetrix® HG-U133A 2.0 microarrays for each cell population. Gene profiles were differentially compared for each population combination.

2014-07-23 | GSE59665 | GEO

Temporal Endogenous Gene Expression Profiles in Response to Lipid-Mediated Transfection

Project description:The current understanding of the molecular factors underlying LF2000-mediated transfection is largely unknown. Cationic LF2000 gene delivery system was used to transfer GFP transgene to HEK293T cells. FACS separation of transfected (GFP positive), untransfected (GFP negative), and untreated cells enabled gene expression profiles to be obtained using Affymetrix® HG-U133A 2.0 microarrays for each cell population. Gene profiles were differentially compared for each population combination.

2014-07-23 | GSE59666 | GEO

Expression data from NKYS transfected with siRNAs or plasmids

Project description:The siRNA transfection includes JAK3 and EZH2 siRNAs. The plasmid transfection includes EZH2 WT and its mutants. We used gene expression data from JAK3 siRNA to test whether it affected epigenetic regulation, and used EZH2 mutants to study how it affected EZH2 canonical function.

2016-07-26 | GSE75680 | GEO

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