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MRNA expression profile modified by microRNA mir-517a (MIR517A) in human hepatocellular carcinoma cell line

ABSTRACT: mRNA expression profile modified by stable transfection of microRNA mir-517a (MIR517A) in a human hepatocellular carcinoma cell line Huh-7 Keywords: Hepatocellular carcinoma, Expression array, microRNA microRNA mir-517a (MIR517A) was transfected to Huh-7 cells using GFP-expressing lentiviral vector. Infected cells were selected using flow cytometry and subjected to mRNA expression microarray experiment. The profiles were compared to controls cells infected with only GFP protein.

ORGANISM(S): Homo sapiens

SUBMITTER: Yujin Hoshida

PROVIDER: E-GEOD-20679 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MicroRNA-based classification of hepatocellular carcinoma and oncogenic role of miR-517a.

Toffanin Sara S Hoshida Yujin Y Lachenmayer Anja A Villanueva Augusto A Cabellos Laia L Minguez Beatriz B Savic Radoslav R Ward Stephen C SC Thung Swan S Chiang Derek Y DY Alsinet Clara C Tovar Victoria V Roayaie Sasan S Schwartz Myron M Bruix Jordi J Waxman Samuel S Friedman Scott L SL Golub Todd T Mazzaferro Vincenzo V Llovet Josep M JM

Gastroenterology 20110213 5

<h4>Background & aims</h4>Hepatocellular carcinoma (HCC) is a heterogeneous tumor that develops via activation of multiple pathways and molecular alterations. It has been a challenge to identify molecular classes of HCC and design treatment strategies for each specific subtype. MicroRNAs (miRNAs) are involved in HCC pathogenesis, and their expression profiles have been used to classify cancers. We analyzed miRNA expression in human HCC samples to identify molecular subclasses and oncogenic miRNA ...[more]

PMID: 21324318

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MicroRNA-Based Classification of Hepatocellular Carcinoma and Oncogenic Role of miR-517a

Project description:Hepatocellular carcinoma (HCC) is a complex and heterogeneous tumor due to activation of multiple cellular pathways and molecular alterations. Herein, we report the first molecular classification of 89 HCC based on the expression of 358 microRNAs and integrative genomic analysis. Three main subclasses of HCC were identified : two of them were associated with beta-catenin mutations or aggressive phenotype. A subset of the subclass of aggressive tumors (8/89, 9%) showed overexpression of a cluster of microRNAs located on chr19q13.41 (C19MC locus. We showed that miR 517a, representing C19MC, promoted cell proliferation, migration and invasion in vitro and induced the development of aggressive tumors in vivo suggesting its role as a novel oncogenic driver in HCC. This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2011-02-17 | E-GEOD-20596 | biostudies-arrayexpress

mRNA expression profile modified by microRNA mir-517a (MIR517A) in human hepatocellular carcinoma cell line

Project description:mRNA expression profile modified by stable transfection of microRNA mir-517a (MIR517A) in a human hepatocellular carcinoma cell line Huh-7 Keywords: Hepatocellular carcinoma, Expression array, microRNA

2011-02-17 | GSE20679 | GEO

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2019-04-24 | PXD013054 | Pride

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2014-10-09 | PXD001339 | Pride

Human hepatocarcinoma cells : Huh-7 vs. SNU-739

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