Unknown,Transcriptomics,Genomics,Proteomics

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Profiling differentially expressed earthworm genes in response to TNT, RDX, or TNT+RDX exposure.


ABSTRACT: To understand molecular mechanisms of the joint effects of 2,4,6-trinitrotoluene (TNT) and hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), both widely used ordnance compounds, we constructed a microarray consisting of 4,032 cDNA isolated from the earthworm Eisenia fetida using the suppressive subtractive hybridization technique. Worms were exposed to TNT-, RDX-, or TNT+RDX-spiked soil for 28 days (TNT 50 mg/kg, RDX 30 mg/kg). Keywords: Combined toxicity of TNT and RDX to earthworm (Eisenia fetida) We analyzed 40 arrays for 4 treatments (control, TNT 50ppm, RDX 30ppm, TNT 50ppm + RDX 30ppm) with 5 biological replicates per treatment using an interwoven loop design.

ORGANISM(S): Eisenia fetida

SUBMITTER: Ping Gong 

PROVIDER: E-GEOD-8909 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptomic analysis of RDX and TNT interactive sublethal effects in the earthworm Eisenia fetida.

Gong Ping P   Guan Xin X   Inouye Laura S LS   Deng Youping Y   Pirooznia Mehdi M   Perkins Edward J EJ  

BMC genomics 20080101


<h4>Background</h4>Explosive compounds such as TNT and RDX are recalcitrant contaminants often found co-existing in the environment. In order to understand the joint effects of TNT and RDX on earthworms, an important ecological and bioindicator species at the molecular level, we sampled worms (Eisenia fetida) exposed singly or jointly to TNT (50 mg/kg soil) and RDX (30 mg/kg soil) for 28 days and profiled gene expression in an interwoven loop designed microarray experiment using a 4k-cDNA array.  ...[more]

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