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Transcription profiling of human airway epithelial cells metapneumovirus-induced gene networks

ABSTRACT: This experiment is designed to study the effects to HMPV on A549 over time Experiment Overall Design: Confluent monolayers of A549 cells were infected with hMPV at MOI of 1 in serum-free media and harvested at 6, 12, 24, 48, or 72 hours post-infection to extract total RNA using RNAqueousÂ®-Midi Kit (Ambion, Austin, TX), according to manufacturerâs instruction;3 Affymetrix HG-U133 plus 2.0 were used for hybridization.

ORGANISM(S): Homo sapiens

SUBMITTER: Antonella Casola

PROVIDER: E-GEOD-8961 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of human metapneumovirus-induced gene networks in airway epithelial cells by microarray analysis.

Bao X X Sinha M M Liu T T Hong C C Luxon B A BA Garofalo R P RP Casola A A

Virology 20080129 1

Human metapneumovirus (hMPV) is a major cause of lower respiratory tract infections in infants, elderly and immunocompromised patients. Little is known about the response to hMPV infection of airway epithelial cells, which play a pivotal role in initiating and shaping innate and adaptive immune responses. In this study, we analyzed the transcriptional profiles of airway epithelial cells infected with hMPV using high-density oligonucleotide microarrays. Of the 47,400 transcripts and variants repr ...[more]

PMID: 18234263

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