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Rotating wall vessel (RWV) grown A549s compared to monolayer grown A549s infected with F. tularensis SchuS4 over a 22 hour time-course (2, 6 and 22 h post infection)

ABSTRACT: A549 epithelial cells grown under two different conditions were compared. A549s grown in rotating wall vessel cultures display a more in-vivo like phenotype. The data here describe the transcriptional differences between the two culture methods when infected with Francisella tularensis SchuS4 over a 22 hour time-course 1 colour; Cy3 labelled, 6 biological replicates for each culture condition and time-point, A549s grown in RWVs (RWV) compared to A549 grown in monolayers (mono), Infected vs. NaM-CM-/ve

ORGANISM(S): Homo sapiens

SUBMITTER: Jonathan David

PROVIDER: E-GEOD-40808 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Rotating wall vessel (RWV) grown A549s compared to monolayer grown A549s infected with F. tularensis SchuS4 over a 22 hour time-course (2, 6 and 22 h post infection)

Project description:A549 epithelial cells grown under two different conditions were compared. A549s grown in rotating wall vessel cultures display a more in-vivo like phenotype. The data here describe the transcriptional differences between the two culture methods when infected with Francisella tularensis SchuS4 over a 22 hour time-course

2012-12-01 | GSE40808 | GEO

Rotating wall vessel (RWV) grown A549s compared to monolayer grown A549s

Project description:A549 epithelial cells grown under two different conditions were compared. A549s grown in rotating wall vessel cultures disp[lay a more in-vivo like phenotype. The data here describe the transcriptional differences between the two culture methods 1 colour; Cy3 labelled, 5 biological replicates for each culture condition, A549s grown in RWVs (RWV) compared to A549 grown in monolayers (mono)

2012-10-01 | E-GEOD-36931 | biostudies-arrayexpress

Rotating wall vessel (RWV) grown A549s compared to monolayer grown A549s

2012-10-01 | GSE36931 | GEO

Transcription profiling of Francisella tularensis LVS wild type and hfq mutant strains grown on solid medium

Project description:Comparison of Francisella tularensis LVS wild-type and hfq mutant strains grown on chocolate agar plates.

2009-05-01 | E-MEXP-1884 | biostudies-arrayexpress

Transcription profiling of Francisella tularensis LVS wild type and hfq mutant strains grown on complex liquid medium

Project description:Comparison of Francisella tularensis LVS wild-type and hfq mutant strains grown in complex liquid broth.

2009-05-01 | E-MEXP-1891 | biostudies-arrayexpress

Transcription profiling of Francisella tularensis LVS wild type and hfq mutant strains grown on defined liquid medium (Chamberlains medium)

Project description:Comparison of Francisella tularensis LVS wild-type and hfq mutant strains grown in defined liquid medium (Chamberlain's medium)

2009-05-01 | E-MEXP-1892 | biostudies-arrayexpress

Gene expression data from F. tularensis-exposed neutrophils

Project description:We demonstrated recently that both constitutive and FAS-triggered apoptosis of human neutrophils are profoundly impaired by Francisella tularensis, but how this is achieved is largely unknown. To test the hypothesis that changes in neutrophil gene expression contribute to this phenotype, we used human oligonucleotide microarrays to identify differentially regulated genes in cells infected with F. tularensis strain LVS compared with uninfected controls. In order to examine the effect of F. tularensis on the neutrophil transcriptome, we performed microarray expression analysis on human neutrophils treated with F. tularensis subsp. holarctica live vaccine strain (LVS). Polymorphonuclear leukocytes (PMNs) were isolated from the blood of healthy donors. Control and F. tularensis-exposed PMNs were incubated at 37C for 0, 3, 6, 12, 24, and 48 hours.

2012-10-22 | E-GEOD-37416 | biostudies-arrayexpress

Francisella tularensis subsp. tularensis Induces a Unique Pulmonary Inflammatory Response: Role of Bacterial Gene Expression in Temporal Regulation of Host Defense Responses

Project description:Virulent Francisella tularensis induces a unique pulmonary inflammatory response characterized by temporal regulation of innate immune pathways correlating with altered bacterial gene expression patterns. The pulmonary transcriptional response to aerosolized virulent F. tularensis was compared to other lethal and non-lethal respiratory pathogens.

2013-04-15 | E-GEOD-44320 | biostudies-arrayexpress

Transcription profiling of Francisella tularensis LVS carrying plasmid pFNLTP6-gro or pFNLTP-gro-0851 (expressing rpoH) grown to mid exponential phase

Project description:F. tularensis LVS carrying plasmid pFNLTP6-gro or pFNLTP-gro-0851 (expressing rpoH) were grown to mid exponential phase in Schaedler + vitamin K3 medium and RNA isolated.

2009-07-01 | E-MEXP-2048 | biostudies-arrayexpress

Whole-genome in vitro expression analysis of F. tularensis isolates grown to mid log phase

Project description:This study was aimed to perform comparative expression analysis of both virulent and avirulent strains of F. tularensis with a goal to possibly identify genes unique to low and high virulent strains to understand their role, if any in virulence features of an isolate. Initially, thirty strains were selected representing both type A and type B clades of F. tularensis. Five strains failed to grow on Chamberlain's medium (Chamberlain 1965) and were excluded from the study. All the isolates were grown to mid-log phase in Chamberlain’s medium and the total RNA was collected by standard protocols for expression analysis. The samples were analyzed in duplicates. F. tularensis Schu S4, a known virulent strains was used as a reference strain for this study. Chamberlain RE (1965) "Evaluation of Live Tularemia Vaccine Prepared in a Chemically Defined Medium," Applied Microbiology, 13(2): 232-5

2011-02-28 | GSE26048 | GEO

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