Unknown,Transcriptomics,Genomics,Proteomics

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Analysis of pvalb-egfp cells from mouse visual cortex.


ABSTRACT: We provide an annotated cDNA clone collection which is particularly suitable for transcriptomic analysis in the mouse brain. Using it on microarrays, we compared the transcriptome of EGFP positive and negative cells in a parvalbumin-egfp transgenic background and showed that more than 30 % of clones are differentially expressed. Our clone collection will be a useful resource for the study of the transcriptome of single cell types. Keywords: Cell type comparison Comparison between fluorescent and non-fluorescent cells isolated from the visual cortex of parvalbumin-egfp transgenic mice. Using five biological replicates with a dye-swap strategy (10 hybridisations).

ORGANISM(S): Mus musculus

SUBMITTER: Charles Plessy 

PROVIDER: E-GEOD-8968 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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<h4>Background</h4>The transcriptome of the cerebral cortex is remarkably homogeneous, with variations being stronger between individuals than between areas. It is thought that due to the presence of many distinct cell types, differences within one cell population will be averaged with the noise from others. Studies of sorted cells expressing the same transgene have shown that cell populations can be distinguished according to their transcriptional profile.<h4>Methodology</h4>We have prepared a  ...[more]

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