Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of mouse v-abl-transformed pre-B cells were treated with 3 uM STI571 for 48 hours


ABSTRACT: The objective is to identify genes that are differentially expressed following the introduction of DNA double strand breaks (DSBs) by the Rag proteins in murine pre-B cells. Cells lacking Artemis are used since the Rag-induced DSBs will not be repaired and, thus, will provide a continuous stimulus to the cell. Cells lacking Artemis and Atm are used to determine which gene expression changes depend on Atm and cells lacking Artemis that express an Iï?«Bï?¡ dominant negative are used to determine which gene expression changes depend on NFkB. Experiment Overall Design: Murine v-abl-transformed pre-B cells were treated with 3 uM STI571 for 48 hours. Cell types included Wild type (3 biological replicates), RAG-2-deficient (3 biological replicates), Artemis-deficient (3 biological replicates), Artemis and ATM-deficient (2 biological replicates, each with a technical replicate), and Artemis-deficient expressing dominant negative IkB (3 biological replicates). Each sample was hybridized once using Affymetrix Mouse Genome 2.0 GeneChip arrays (Mouse 430 v2, Affymetrix, Santa Clara, CA). Data were analyzed using the RAG-2-deficient samples as the controls. Additionally, purified bone marrow pre-B cells were harvested from RAG-1-deficient (2 biological replicates) and Artemis-deficient (2 biological replicates) mice with an IgH transgene. Each sample was hybridized once using Affymetrix Mouse Genome 2.0 GeneChip arrays (Mouse 430 v2, Affymetrix, Santa Clara, CA). Data were analyzed using the RAG-1-deficient:IgH samples as the controls.

ORGANISM(S): Mus musculus

SUBMITTER: NIEHS Microarray Core 

PROVIDER: E-GEOD-9024 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2008-10-12 | GSE9024 | GEO
2014-06-18 | E-GEOD-58599 | biostudies-arrayexpress
2016-01-13 | E-GEOD-67854 | biostudies-arrayexpress
2013-03-13 | E-GEOD-38044 | biostudies-arrayexpress
2014-06-18 | GSE58599 | GEO
2016-01-13 | GSE67854 | GEO
2013-03-13 | GSE38044 | GEO
2019-08-22 | GSE129129 | GEO
2019-08-22 | GSE129124 | GEO
2019-12-23 | GSE133954 | GEO