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Transcription profiling of Arabidopsis LEAFY mutant seedlings treated with dexamethasone and/or cycloheximide

ABSTRACT: Global analysis of gene expression in 9 day old LEAFY-GR, 35S::LFY or Landsberg erecta seedlings treated with the steroid dexamethasone and/or the protein synthesis inhibitor cycloheximide.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Doris Wagner

PROVIDER: E-GEOD-911 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genomic identification of direct target genes of LEAFY.

William Dilusha A DA Su Yanhui Y Smith Michael R MR Lu Meina M Baldwin Don A DA Wagner Doris D

Proceedings of the National Academy of Sciences of the United States of America 20040121 6

The switch from vegetative to reproductive development in plants necessitates a switch in the developmental program of the descendents of the stem cells in the shoot apical meristem. Genetic and molecular investigations have demonstrated that the plant-specific transcription factor and meristem identity regulator LEAFY (LFY) controls this developmental transition by inducing expression of a second transcription factor, APETALA1, and by regulating the expression of additional, as yet unknown, gen ...[more]

PMID: 14736918

Similar Datasets

Identification of LEAFY targets during reproductive transition

Project description:Global analysis of gene expression in 9 day old LEAFY-GR, 35S::LFY or Landsberg erecta seedlings treated with the steroid dexamethasone and/or the protein synthesis inhibitor cycloheximide. Keywords: other

2003-12-18 | GSE911 | GEO

LFY is pioneer transcription factor [MNase-seq]

Project description:This study identified LEAFY (LFY) as a pioneer transcription factor. We made use of 35S:LFY-GR, an inducible version of the LFY protein fused to the rat glucocorticoid hormone binding domain. In root explants, steroid activated LFY-GR triggers synchronous and abundant flower induction (PMID: 15225291). We combined LFY ChIP-seq, MNase-seq before and after LFY binding and time-course RNA-seq in 35S:LFY-GR root explants to characterize the role of LFY as a pioneer transcription factor.

2020-12-15 | GSE141705 | GEO

LFY is pioneer transcription factor [ChIP-seq]

2020-10-27 | GSE141704 | GEO

LFY is pioneer transcription factor [RNA-seq]

2020-12-15 | GSE141703 | GEO

Flower development

Project description:Wild type and mutanat Arabiposis plants grown in short days (9L:15D) for 30 days at 21°C, then shifted to long days (16L:8D). Genotypes: Columbia wild type (Col-0) Landsberg erecta (Ler) leafy-12 (lfy-12, in Col-0) constans-2 (co-2, in Ler) flowering locus T-2 (ft-2, in Ler) Time points: 0, 3, 5, and 7 days after shift to long days Keywords = flowering Keywords: time-course

2003-08-06 | GSE576 | GEO

LEAFY target genes reveal a link between flower development and biotic stimulus response (III)

Project description:The transition from vegetative growth to flower formation is critical for the survival of flowering plants. The plant-specific transcription factor LEAFY (LFY) has central, evolutionarily conserved roles in this process, both in the formation of the first flower and later in floral patterning. We performed genome-wide binding and expression studies to elucidate the molecular mechanisms by which LFY executes these roles. Our study reveals that LFY directs an intricate regulatory network in control of floral homeotic gene expression and, unexpectedly, controls the expression of genes regulating the response to external stimuli in Arabidopsis. We further show that LFY dampens responses to a bacterial MAMP (microbe-associated molecular pattern) and to pathogen challenge. Our findings suggest a molecular mechanism for the coordination of reproductive stage development and disease response programs in plants. Regulation of these distinct survival programs by a single transcription factor may ensure optimal allocation of plant resources for reproductive fitness. Expression array analysis used to identify genes differentially expressed upon LFY induction in 9-day-old shoot apices. Expression array analysis of 9-day-old 35S::LFY-GR dexamethasone-treated seedlings compared to 9-day-old WT dexamethasone-treated seedlings. Four biological replicate samples.

2011-05-11 | E-GEOD-28062 | biostudies-arrayexpress

Transcription profiling time series of Arabidopsis floral induction pathway mutants following shift from short to long day length

Project description:Wild type and mutanat Arabiposis plants grown in short days (9L:15D) for 30 days at 21Â°C, then shifted to long days (16L:8D).,Genotypes:,Columbia wild type (Col-0),Landsberg erecta (Ler),leafy-12 (lfy-12, in Col-0),constans-2 (co-2, in Ler),flowering locus T-2 (ft-2, in Ler),Time points:,0, 3, 5, and 7 days after shift to long days

2007-07-10 | E-GEOD-576 | biostudies-arrayexpress

LEAFY-GR time-course experiment in callus

Project description:Callus derived from 35S:LFY-GR plants was treated with dexamethasone- or was mock-treated for 0,2,4,6,8,10,12, or 22 hours. Four biologically independent sets of samples were generated and RNA probes derived from these samples were co-hybridized to a cDNA array enriched for flower-specific transcripts. The data were analyzed with Rosetta Resolver as described in Wagner et al. (2004). Keywords: time-course

2004-07-02 | GSE1411 | GEO

Transcription factor Interplay between LEAFY and APETALA1/ CAULIFLOWER during Floral Initiation

Project description:The transcription factors LEAFY (LFY) and APETALA1 (AP1)_together with the AP1 paralog CAULIFLOWER (CAL)_control the onRep_of flower development in a partially redundant manner. This redundancy is thought to be mediated_at least in part_through the regulation of a shared Rep_of target genes. However_whether these genes are independently or cooperatively regulated by LFY and AP1/CAL_is currently unknown. To better understand the regulatory relationship between LFY and AP1/CAL during floral initiation_we monitored the activity of LFY in the absence of AP1/CAL function. We found that the regulation of several known LFY target genes is unaffected by AP1/CAL perturbation_while others appear to require AP1/CAL activity. Furthermore_we obtained evidence that LFY and AP1/CAL control the expression of some genes in an antagonistic manner. Notably_these include key regulators of floral initiation such TERMINAL FLOWER1 (TFL1)_which had been previously reported to be directly repressed by both LFY and AP1. We show here that TFL1 expression is suppressed by AP1 but promoted by LFY. We further demonstrate that LFY has an inhibitory effect on flower formation in the absence of AP1/CAL activity. We propose that LFY and AP1/CAL may act as part of an incoherent feed-forward loop to control the establishment of a stable developmental program for the formation of flowers.

2017-04-28 | GSE96799 | GEO

A biophysical DNA binding model for the LEAFY transcription factor reveals the evolutionary fluidity of its binding sites

Project description:Despite great advances in sequencing capacity, generating functional information for non-model organisms remains a challenge. One solution lies in an improved ability to predict genetic circuits based on primary DNA sequence combined with the characterization of regulatory molecules from model species. Here, we focus on the LEAFY (LFY) transcription factor, a conserved master regulator of floral development. Starting with biochemical and structural information, we built a biophysical model describing LFY DNA binding specificity in vitro that accurately predicts in vivo LFY binding sites in the Arabidopsis thaliana genome. Extending the model to other species, we show that it can correctly identify functional homologs of known LFY targets from Arabidopsis thaliana in other angiosperms, even if a functional shift between orthologs and paralogs has occurred. Moreover, this model demonstrates the evolutionary fluidity of the link between LFY and one of its target genes, underlining how this regulatory interaction can be conserved despite changes in position, sequence and affinity of the LFY binding sites. Our study shows that the cis-element fluidity recently illustrated in animals also exists in plants, and that it can be detected without any experimental work in each individual species, using a biophysical transcription factor model. A. thaliana LEAFY ChIP-seq w control, 2 replicates

2011-05-13 | E-GEOD-24568 | biostudies-arrayexpress

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