Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Xenopus hybrid male in sex reversal experiment


ABSTRACT: Gene expression was examined in genetic females that were sex-reversed to be phenotypically male compared to normal hybrid males; We used microarrays to test expression in the gonad of sex-reversed males (ZW) compared to normal males (ZZ) Experiment Overall Design: RNA from testis of sexually mature individuals was extracted and hybridized onto Affymetrix microarrays.

ORGANISM(S): Xenopus laevis x Xenopus muelleri

SUBMITTER: John Malone 

PROVIDER: E-GEOD-9505 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Physiological sex predicts hybrid sterility regardless of genotype.

Malone John H JH   Michalak P P  

Science (New York, N.Y.) 20080101 5859


Reproductive isolation between biological species is characterized by Haldane's rule, which states that the heterogametic sex (XY or ZW) suffers the most dysfunctional effects of hybridization. It has been hypothesized that, in addition to X-linked recessive genes, Haldane's rule also reflects the faster evolution of genes related to male reproduction (faster-male evolution). We used sex-reversed hybrid Xenopus to test faster-male evolution by examining the fertility of sex-reversed individuals  ...[more]

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