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Transcription profiling of human brain Total RNA vs Universal Human Reference RNA on 4 different commercially available microarrays

ABSTRACT: Commercially available human genomic microarrays from four different manufacturers were used to compare Human Brain Total RNA against Universal Human Reference RNA (both commercially available) prepared at two different starting amounts (20 µg or 1µg). For each amount of RNA, 6 replicates were performed with Human Brain Total RNA labelled with Cy3, and Universal Human Reference RNA labelled with Cy5. The labelling was then reversed (dye flip) creating another 6 replicates. This meant that for each of the four manufacturers there were a total of 24 arrays. Image processing was performed with two different software packages, and data was normalized with three different strategies. In addition to the spotted arrays, assays were also performed with the same RNA starting material using in situ synthesised oligonucleotide arrays (human genome arrays). These were carried out in two independent laboratories. The first prepared 12 arrays (6 replicates) with 10µg of RNA, the second lab used a range of RNA starting amounts of either 10µg, 1.0µg or 0.1µg, and prepared 12 arrays for each amount. In total, therefore, there were 48 in situ synthesised oligonucleotide arrays

ORGANISM(S): Homo sapiens

SUBMITTER: Daniel Hopkins

PROVIDER: E-LGCL-5 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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