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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Chromatin immunoprecipitation of mouse tumour cell line L1210 synchronized and sampled after mitosis to map replication time zones in the mouse genome

ABSTRACT: Measuring replication time of regions in teh mouse genome, using a novel synchronization method, labeling of BrdU, immunoprecipitation and hybridization to microarrays

INSTRUMENT(S): G2565AA DNA microarray scanner [Agilent]

ORGANISM(S): Mus musculus

SUBMITTER: Shlomit Farkash-Amar

PROVIDER: E-MEXP-1022 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global organization of replication time zones of the mouse genome.

Farkash-Amar Shlomit S Lipson Doron D Polten Andreas A Goren Alon A Helmstetter Charles C Yakhini Zohar Z Simon Itamar I

Genome research 20080730 10

The division of genomes into distinct replication time zones has long been established. However, an in-depth understanding of their organization and their relationship to transcription is incomplete. Taking advantage of a novel synchronization method ("baby machine") and of genomic DNA microarrays, we have, for the first time, mapped replication times of the entire mouse genome at a high temporal resolution. Our data revealed that although most of the genome has a distinct time of replication ei ...[more]

PMID: 18669478

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