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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse lung fibroblasts and Lewis lung cells to investigate the transciptional regulation that governs cell-cell fusion process

ABSTRACT: This study aimed to investigate the transciptional regulation that governs cell-cell fusion process.

ORGANISM(S): Mus musculus

SUBMITTER: Amir Abdollahi

PROVIDER: E-TABM-381 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of human dermal fibroblasts and lung microvascular endothelial cells irradiated with iron ions

Project description:Normal human dermal fibroblasts (NHDF) and human lung microvascular endothelial cells (HMVEC-L) were irradiated with iron ions (0, 0.2, 0.4 and 1 Gy, 1GEv/n) at Brookhaven National Labs (BNL). Aim of the study is to find differentially transcribed genes in dependance of radiation dose/source and cell type.

2009-04-25 | E-TABM-374 | biostudies-arrayexpress

Transcription profiling of human dermal fibroblasts and lung microvascular endothelial cells irradiated with protons

Project description:Normal human dermal fibroblasts (NHDF) and human lung microvascular endothelial cells (HMVEC-L) were irradiated with protons (0, 0.5, 1 and 2 Gy, 1GEv/n) at Brookhaven National Labs (BNL). Aim of the study is to find differentially transcribed genes in dependence of radiation dose/source and cell type.

2009-04-25 | E-TABM-379 | biostudies-arrayexpress

Transcription profiling of wild type, MKK3 transfected and Ras transfected mouse fibroblasts which have invaded or not invaded through a separating membrane

Project description:Invasiveness of genetically modified cells is tested. Four cells lines (NIH3T3 untreated control; NIH-Ras positive control which is invasion +; NIH-MKK3actK4 and NIH-MKK3actATN, two constructs with MKK3 gene which shall be investigated for theit invasiveness). Cells on top as well as cells from bottom of separating membrane (those which had "invaded") form all 4 cell lines are collected and expression profiled. Aim is to find genes which are correlated to "invasion".

2009-09-18 | E-TABM-561 | biostudies-arrayexpress

Transcription profiling of human blood from patients with acute kidney injury following open-heart surgery with cardiopulmonary bypass

Project description:Acute kidney Injury (AKI) following open-heart surgery with cardiopulmonary bypass (CPB) is associated with an increased morbidity and mortality. The goal of this study is to explore patterns of gene expression in human AKI following CPB. This prospective observational study was approved by the Institutional Review Board Human Subjects Committee. Design and methods: Peripheral blood RNA samples were collected prospectively from a cohort of 30 patients at Caritas St. Elizabeths Medical Center in Boston, MA, undergoing CPB at time points immediately prior, and two and 24 hours following CPB. Gene expression patterns of four patients who developed AKI (cases) following CPB will be compared with six patients matched for clinical characteristics, who did not develop AKI (controls). AKI was defined by an incremental increase in serum creatinine of 30% within 72 hours following CPB. For peripheral blood RNA sampling, 2.5 ml of venous whole blood was collected into PAXgene Blood RNA Tube collection tubes (PreAnalytiX GmbH, Hombrechtikon, CH). Whole blood RNA was extracted with the PAXgene Blood RNA extraction Kit according to the manufacturers instructions (PAXgene RNA Kit Handbook, 06/2005). All biological samples are stored at 80° C.

2009-12-12 | E-TABM-595 | biostudies-arrayexpress

Transcription profiling of Aspergillus fumigatus isolates following laboratory culture or initiation of infection in the neutropenic murine lung to understand how metabolic and nutritional factors governing adaptation to the host niche contribute to virulence

Project description:To understand how metabolic and nutritional factors governing adaptation to the host niche contribute to the virulence of Aspergillus fumigatus we compared transcriptomes of developmentally matched A.fumigatus isolates following laboratory culture or initiation of infection in the neutropenic murine lung.

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2009-06-06 | E-TABM-659 | biostudies-arrayexpress

Transcription profiling of human carcinoma in situs cells from testis compared to fetal gonocytes, oogonia and cultured embryonic stem cells

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2009-06-15 | E-TABM-488 | biostudies-arrayexpress

Transcription profiling of irradiated human dermal microvascular endothelial cells

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2010-01-13 | E-TABM-617 | biostudies-arrayexpress

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2009-03-03 | E-TABM-265 | biostudies-arrayexpress

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Project description:genomic comparison of group I C.botulinum strains

2009-03-26 | E-TABM-309 | biostudies-arrayexpress