Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of rat spinal cord with experimental autoimmune encephalomyelitis at 3 disease points, acute, relapsing and recovering vs. controls

ABSTRACT: The intention of the study was to examine the spinal cord gene expression at three different EAE disease points and to compare them with three nonimmunised, healthy rats. We immunised female DA rats with recombinant MOG-protein and sacrificed them during the acute phase of EAE defined as the first EAE attack leading to a clinical score of at least three, in the recovery phase in which the rats have begun to gain weight after the acute phase and in the relapsing phase - rats belonging to this group have been sacrificed while they were in the peak of the first relaps. Three healthy control rats, three from the acute phase of EAE (day 11 and 12 after EAE induction), three from the recovery phase (day 13 and 14 after EAE induction) and four rats from the relapsing phase (day 24 and 25 after EAE induction) have been finally sacrificed for the study.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Andre Mueller

PROVIDER: E-MEXP-1025 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Publications

Novel role for SLPI in MOG-induced EAE revealed by spinal cord expression analysis.

Mueller Andre M AM Pedré Xiomara X Stempfl Thomas T Kleiter Ingo I Couillard-Despres Sebastien S Aigner Ludwig L Giegerich Gerhard G Steinbrecher Andreas A

Journal of neuroinflammation 20080526

<h4>Background</h4>Experimental autoimmune encephalomyelitis (EAE) induced by myelin oligodendrocyte protein (MOG) in female Dark Agouti (DA) rats is a chronic demyelinating animal model of multiple sclerosis (MS). To identify new candidate molecules involved in the evolution or repair of EAE-lesions we used Affymetrix oligonucleotide microarrays to compare the spinal cord transcriptome at the peak of EAE, during remission and at the first relapse with healthy DA rats.<h4>Methods</h4>Untreated D ...[more]

PMID: 18501024

Similar Datasets

Project description:Neural progenitor cells (NPCs) have regenerative capabilities that are activated during inflammation. By measuring the global transcriptome and performing functional studies, we aimed at elucidating if and how NPCs from the non-germinal niche of the spinal cord differ from germinal niche NPCs, here represented by the subventricular zone (SVZ) NPCs. Moreover, we investigated how these cells are affected by chronic inflammation modeled by Experimental Autoimmune Encephalomyelitis (EAE). NPCs were isolated and propagated from the SVZ and cervical, thoracic and caudal regions of the spinal cord from healthy rats and rats subjected to EAE. Using Affymetrix microarray analyses, the global transcriptome was measured in the different NPC populations both in undifferentiated and differentiated cultures. These analyses were paralleled by differentiation studies and quantitative RT-PCR of differentiation-specific genes. In NPCs isolated from healthy rats, transcriptional and functional analyses revealed a higher neurogenic potential in SVZ-derived NPCs compared to spinal cord NPCs. The neurogenicity of spinal cord NPCs was increased by exposure to the inflammatory environment. Concurrently, their gliogenicity was decreased which was supported by a decreased expression of glial differentiation signature genes and related signaling pathways. Differentiation analyses showed that spinal cord NPCs from EAE rats generated fewer oligodendrocytes and astrocytes than NPCs isolated from healthy controls. 54 samples were analysed. The transcriptome of NPCs isolated from healthy rats or rats with EAE was measured. The NPCs were isolated from the SVZ and the cervical, thoracic and caudal parts of the spinal cord. Pair wise student t-test analysis between the 3 EAE replicates and naive controls for each respective tissue type was performed. Genes with a signal value above the cut-off of 50 and with FDR M-bM-^IM-$ 5% and a foldchange of M-bM-^IM-%1.2 or M-bM-^IM-$-1.2 were selected for further analysis.

Dataset Information

Transcription profiling of rat spinal cord with experimental autoimmune encephalomyelitis at 3 disease points, acute, relapsing and recovering vs. controls

Publications

Novel role for SLPI in MOG-induced EAE revealed by spinal cord expression analysis.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets