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Transcriptome profiling of a backcross between experimental autoimmune encephalomyelitis (EAE)-susceptible DA and EAE-resistant PVG rat strains to identify expression quantitative trait loci.

ABSTRACT: The aim of the experiment is to characterize the genetics and mechanisms of the inflammatory response after induction of experimental autoimmune encephalomyelitis (EAE), a model of multiple sclerosis, by studying the genome wide expression in the spleen in late disease. A backcross was created between EAE-susceptible DA and EAE-resistant PVG rats. At day 35 after induction of EAE with myelin oligodendrocyte glycoprotein (MOG) in these rats, spleens were taken for transcriptional profiling.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Melanie Thessen Hedreul

PROVIDER: E-MTAB-784 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Combining genetic mapping with genome-wide expression in experimental autoimmune encephalomyelitis highlights a gene network enriched for T cell functions and candidate genes regulating autoimmunity.

Thessen Hedreul Melanie M Möller Steffen S Stridh Pernilla P Gupta Yask Y Gillett Alan A Daniel Beyeen Amennai A Öckinger Johan J Flytzani Sevasti S Diez Margarita M Olsson Tomas T Jagodic Maja M

Human molecular genetics 20130729 24

The experimental autoimmune encephalomyelitis (EAE) is an autoimmune disease of the central nervous system commonly used to study multiple sclerosis (MS). We combined clinical EAE phenotypes with genome-wide expression profiling in spleens from 150 backcross rats between susceptible DA and resistant PVG rat strains during the chronic EAE phase. This enabled correlation of transcripts with genotypes, other transcripts and clinical EAE phenotypes and implicated potential genetic causes and pathway ...[more]

PMID: 23900079

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