Unknown,Transcriptomics,Genomics,Proteomics

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MicroRNA profiling of human breast cancer cell line MCF7 under conditions of hypoxia

ABSTRACT: Hypoxia results in the changes in expression of many genes, the majority of which are mediated via the transcriptional activity of the hypoxia inducible factor (HIF) complex. However, other mechanisms of gene regulation by hypoxia are likely and include control of mRNA stability, regulation of mRNA translation and regulation mediated by micrornas. The aim of this study is to identify microRNAs which expression is regulated by hypoxia. We chose the breast cancer line MCF7 for study as we had previously characterised the expression of the components of the HIF system in that cell line and undertaken an extensive study of the gene expression profile in response to hypoxia, a prolyl hydroxylase inhibitor dimethyloxalylglycine and HIF-1a isoform manipulations (Eldvidge. G.P. et al. (2006) JBC, vol. 281, 22, 15215-15226).

INSTRUMENT(S): GenePix 4000B [Axon Instruments]

ORGANISM(S): Homo sapiens

SUBMITTER: Carme Camps 

PROVIDER: E-MEXP-1111 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

hsa-miR-210 Is induced by hypoxia and is an independent prognostic factor in breast cancer.

Camps Carme C   Buffa Francesca M FM   Colella Stefano S   Moore John J   Sotiriou Christos C   Sheldon Helen H   Harris Adrian L AL   Gleadle Jonathan M JM   Ragoussis Jiannis J  

Clinical cancer research : an official journal of the American Association for Cancer Research 20080301 5

<h4>Purpose</h4>MicroRNA (miRNA) expression alterations have been described in cancer. Many cancers are characterized by areas of hypoxia, enhanced hypoxia-inducible factor (HIF) levels, and increased expression of hypoxically regulated genes, all of which correlate with patient outcome. We examined hypoxia-induced miRNA expression changes to identify markers of survival in breast cancer.<h4>Experimental design</h4>We used microarrays to analyze miRNA expression changes induced by hypoxia in MCF  ...[more]

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