Project description:We used a chicken immune-targeted gene array to analyse the differences in gene expression in the bursa of Fabricius from genetically resistant and susceptible animals infected with Infectious Bursal Disease Virus (IBDV).
Project description:Global analysis of gene expression during development and ripening of citrus fruit flesh. Samples taken from fruit development phases I,II and III (Bain JM, 1958, Aust J Bot, 6: 1-24 ) were compared
Project description:We have applied our research on a well-documented biological model, the epithelial cell evasion mediated by the ectopic expression of the snail protein. Indeed, ectopic expression of Snail, a transcriptional repressor, is widely used to study the initial step of epithelial cell evasion also called Epithelial to Mesenchymal Transition (EMT). During this process, cells loose their intercellular junctions and acquired motility and an invasive behaviour. In order to have a temporal view of the gene expression modulation that occurred during EMT, we have established a biological model based on the conditional expression of snail protein in human non-invasive MCF-7 breast carcinoma cells that do not express endogenous snail and exhibit a well-organized epithelial phenotype. 96 hours after snail induction, the epithelial to mesenchymal cell phenotype conversion is completed.
Project description:To evaluate the experimental performance of Actichip microarrays, a series of 10 repeated hybridisation experiments, including 5 dye swaps, were carried out with optimised target labelling, hybridisation, scanning and data analysis protocols. All the procedures were standardised to limit the impact of experimental bias or biological variations on data. The same series of high quality RNA samples purified from human breast adenocarcinoma MCF-7 cells and from human skeletal muscle was used in our experiments. Epithelial cells and muscle tissue were chosen because they express well-characterised sets of cytoskeleton genes, and were anticipated to give well-contrasted differential expression data when analysed with Actichip.
Project description:double transgenic mice (tTA/IkappaBalpha-AA) were treated with doxycycline (DOX), which represses expression of the transgenic IkappaBalpha. Double transgenic mice without Dox-treatment overexpress the transdominant IkappaBalpha, which represses NFkappaB activation.
Project description:Transcription profiling of the ethylene-induced abscission process in laminar abscission zone enriched tissues and petiole of debladed mature citrus leaf explants. Samples taken at 0, 6, 12, 24 and 36h after ethylene treatment (10 ul/l) were compared.
Project description:We used DNA microarray technology to investigate the cytoskeleton gene expression profile associated with the acquisition of cell resistance to TNF-alpha in breast carcinoma cell line (MCF7) versus sensitive cells (1001).
Project description:To validate Actichip, we compared this microarray with two other well-established oligonucleotide-based platforms. We used 25-mer oligonucleotide commercial chips (Affymetrix, human genome U133A 2.0) and academic arrays prepared with a 70-mer oligonucleotide set (Operon, human whole genome version 2.0). The same series of high quality RNA samples purified from human breast adenocarcinoma MCF-7 cells and from human skeletal muscle was used in our experiments.