Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

45 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of unisexual female Schistosoma mansoni adult worms obtained from male or female mice hosts to investigate the effect of host sex

ABSTRACT: Experiment studying the effect of host sex on unisex adult female S. mansoni worms.

INSTRUMENT(S): Axon- GenePix4000B

ORGANISM(S): Schistosoma mansoni

SUBMITTER: Michael Waisberg

PROVIDER: E-MEXP-1189 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of male Schistosoma mansoni to investigate the effect of sexual maturation on gene expression

Project description:Experiment studying the effect of maturation on gene expression of S. adult male S. mansoni (LE strain).

2007-06-27 | E-MEXP-972 | biostudies-arrayexpress

Transcription profiling of unisexual male Schistosoma mansoni adult worms obtained from male or female mice hosts to investigate the effect of host sex

Project description:Experiment studying the effect of host sex on unisexual male S. mansoni adult worms.

2007-12-01 | E-MEXP-1188 | biostudies-arrayexpress

Transcription profiling of female Schistosoma mansoni from an unisexual infection of mice compared to worms from a mixed sex infection

Project description:In this study we describe the use of DNA microarrays to study the effect of sexual pairing of Schistosoma mansoni in the female gene expression. To accomplish this objective we compared female worms from unisexual infection with worms from mixed sex infection.

2007-06-26 | E-MEXP-805 | biostudies-arrayexpress

A bulk segregant gene expression analysis of a peach population reveals components of the underlying mechanism of the fruit cold response

Project description:Transcript profiling analysis of fruits from a segregating PopDG population, which were grouped/selected according to extreme cold susceptibility and subjected to different times of cold storage

2013-07-01 | E-MEXP-3902 | biostudies-arrayexpress

Extraction of Total RNA from Single Neurons: Mauthner-cell Cytoplasm vs. Whole Brain

Project description:Astatotilapia burtoni males change their startle behavior in accordance with changes in a socially-mediated phenotypic state. When dominant (DOM), males are brightly colored, isolated from the school and startle readily in response to an auditory pulse. When subordinate (SUB), males are cryptically colored, school and startle less frequently. We hypothesize that the difference in startle responsiveness is governed by serotonergic modulation on the command-like Mauthner cells (M-cells). We used the selective serotonin receptor subtype 2 (5HTR2) antagonist ketanserin to show that serotonin receptors can modulate startle frequency and that the behavioral difference correlates with differences in electrophysiological recordings of M-cells. Specifically, SUB males have a higher serotonergic tone and demonstrate, by behavior and electrophysiological properties, increased sensitivity to 5-HT manipulations compared to DOMs. Immunohistochemistry for serotonin is present around the M-cells. Furthermore, we identified serotonin receptor transcripts in A. burtoni and used single-cell transcriptomics to determine the presence or absence of specific receptor subtypes in the M-cells. Microarray analysis of M-cell samples shows that the neuron’s transcriptome is relatively stable in individuals of different social status. These results are consistent with a role for serotonin in modulating the behavioral response to sensory stimuli in an ecologically-relevant manner through inhibitory interneurons, which establish the membrane resistance of the M-cell before the auditory stimulus. Simple loop with dye-swap containing 6 arrays from 2 sources with 3 biological replicates per source (3 M-cell and 3 whole brain samples).

2010-12-23 | E-GEOD-26277 | biostudies-arrayexpress

Estrogen receptor regulation of the social transcriptome

Project description:We investigated the gene regulatory network of the estrogen receptor in the preoptic region of dominant and subordinate male Astatotilapia burtoni. We compared dominant males receiving either an ER antagonist or vehicle control and seperately compared subordinate males receiving either an ER antagonist or control. 8.25% of all genes examined changed expression in DOMs in comparison to only 0.56% in SUBs. Two separate loops (one for dominant males and one for subordinate males) with dye-swap containing 16 arrays each from 2 sources (an ER antagonist treated animal or a vehicle treated animal)

2011-04-13 | E-GEOD-28508 | biostudies-arrayexpress

Eucalyptus xylem diurnal time series

Project description:The daily cycle of night and day affects the behaviour and physiology of almost all living things. At the molecular level, many genes show daily changes in expression levels. To determine whether changes in transcript abundance occur in wood forming tissues of Eucalyptus trees we used a cDNA microarray to examine gene expression levels at roughly four hour intervals throughout the day. Experiments were performed using RNA extracted from two biological replicates - GU (Eucalyptus grandis x E. urophylla) and GC (Eucalyptus grandis x camaldulensis) trees. A loop design was used, linking six time points. A dye swap was incorporated to eliminate dye bias.

2010-06-25 | E-GEOD-11731 | biostudies-arrayexpress

Gallus gallus Control Versus SU5402, LY294002 or U0126 Treated Preingression Epiblast from Gastrula Stage Embryos

Project description:Transcriptional profiling of the preingression epiblast versus lateral epiblast, of preingression epiblast from control embryos treated with the DMSO carrier vehicle (0.5%) versus embryos treated with SU5402, U0126 or LY294002. Embryos were stages 5-6 at time of tissue isolation. The preingression epiblast (E2 region) is the region of epiblast just lateral to the primitive streak. Lateral epiblast is the E3 region. Two condition experiment. Samples for each condition isolated from at least 30 embryos. The experiments used isolated preingression epiblast, which is defined as the region of epiblast adjacent to the primitive streak that will ingress through the primitive streak. Preingression epiblast was isolated by microdissection using tungsten needles from control embryos or embryos treated with SU5402, U0126 or LY294002.

2011-02-20 | E-GEOD-27403 | biostudies-arrayexpress

Gene expression data for worker honey bees experimentally exposed to a dual infection with Deformed wing virus and Nosema ceranae.

Project description:Bees from 3 unrelated colonies were injected with 1ul PBS extract containing 10^9 genome equivalent of Deformed wIng virus (DWV) and/or fed 10Âµl sucrose solution containing 10^5 fresh Nosema ceranae spores. Control bees were injected and fed with an equivalent DWV- and Nosema-free extract respectively). Bees were kept in cages of 21 bees (7 from each colony), and each cage was replicated 5 times per each of the 4 treatments). Bess were kept in an incubator at 30Â°C/50%RH. At day 12 p.i., bees were flash frozen in liquid nitrogen, and stored at -80Â°C. Bee abdomen RNA was sent to Christina Grozinger lab (Penn State, USA). RNA was pooled for 3 abdomens per replicate for 5 replicates per treatment. Arrays were hybridized in a dye-swap loop design.

2017-03-08 | E-MTAB-5508 | biostudies-arrayexpress

Transcription profiling of wild type and At-MYC2 over-expressing Arabidopsis plants

Project description:Transcriptome analysis of 35S:AtMYC2 vs WT (Col-0)

2007-08-23 | E-ATMX-16 | biostudies-arrayexpress