Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

49 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparative genomic hybridization of Bordetella bronchiseptica strains 253 and RB50 to investigate the cya adenylate cyclase toxin (ACT) operon

ABSTRACT: Bordetella bronchiseptica is a gram-negative respiratory pathogen that causes a diverse spectrum of respiratory disease in a wide-range of hosts. We sought to determine if strains of B. bronchiseptica differed in virulence using the mouse model of infection. Mean lethal doses (LD50) of different B. bronchiseptica strains varied widely in the murine model. B. bronchiseptica strain 253 had a LD50 that was 10-fold lower than the prototypical and fully sequenced B. bronchiseptica strain RB50. Using whole genomic transcriptome analysis covering 100% of B. bronchisetpctica strain RB50ÃÂs predicted open reading frames (ORFs), 253 was identified as lacking expression of adenylate cyclase toxin (ACT). Using whole genomic comparative genomic hybridization analysis and whole genome sequencing, we determined that the cya operon, which is required for ACT production, was absent from the 253 genome.

INSTRUMENT(S): GenePix 4000B [Axon Instruments]

ORGANISM(S): Bordetella bronchiseptica

SUBMITTER: Tracy Nicholson

PROVIDER: E-MEXP-1205 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of Bordetella bronchiseptica strains 253 and RB50 to investigate adenylate cyclase toxin (ACT) expression

Project description:Bordetella bronchiseptica is a gram-negative respiratory pathogen that causes a diverse spectrum of respiratory disease in a wide-range of hosts. We sought to determine if strains of B. bronchiseptica differed in virulence using the mouse model of infection. Mean lethal doses (LD50) of different B. bronchiseptica strains varied widely in the murine model. B. bronchiseptica strain 253 had a LD50 that was 10-fold lower than the prototypical and fully sequenced B. bronchiseptica strain RB50. Using whole genomic transcriptome analysis covering 100% of B. bronchisetpctica strain RB50ÃÂÃÂs predicted open reading frames (ORFs), 253 was identified as lacking expression of adenylate cyclase toxin (ACT).

2007-10-01 | E-MEXP-1203 | biostudies-arrayexpress

Comparative genomic hybridization of Bordetella bronchiseptica strains RB50 and 1289

Project description:B. bronchiseptica strains RB50 and 1289 strains were grown in SS broth at 37°C with shaking overnight and genomic DNA was isolated from bacterial cultures using a DNA extraction kit (Qiagen, Valencia, CA) and digested with DpnII. For each labeling reaction, 2 ug of digested genomic DNA was randomly primed using Cy-5 and Cy-3 dye-labeled nucleotides, with BioPrime DNA labeling kits (Invitrogen, Carlsbad, CA) and the two differentially labeled reactions to be compared were combined and hybridized to a B. bronchiseptica RB50 specific long-oligonucleotide microarray.

2008-12-31 | E-MEXP-1737 | biostudies-arrayexpress

T44625-cgh

Project description:comparative genomic hybridization (CGH) analysis was perfromed in order to identify coding regions present in strain T44625 that are either absent or contain a high degree of sequence divergence compared to the RB50 reference strain.

2013-12-01 | E-MEXP-3968 | biostudies-arrayexpress

Transcription profiling of Bordetella bronchiseptica strains RM50 and 1289

Project description:B. bronchiseptica strains RB50 and 1289 were grown in SS broth, subcultured at a starting OD600 of 0.02 into 50mL of SS broth, grown at 37C for 24 hours while shaking and harvested in log phase (OD600 1.0). Total RNA was extracted with Trizol (Invitrogen, Carlsbad, CA), treated with RNase-free DNase I (Invitrogen, Carlsbad, CA) and purified using RNeasy columns (Qiagen, Valencia, CA) according to the manufacturers instructions. RNA was isolated from two independent biological replicates of strains RB50 and 1289. A 2-color hybridization format was used and dye-swap experiments were performed. For each reaction, 5ug of cDNA was fluorescently labeled. The two differentially labeled reactions to be compared were combined and hybridized to a B. bronchiseptica strain RB50 specific long-oligonucleotide microarray.

2009-01-01 | E-MEXP-1736 | biostudies-arrayexpress

Transcription profiling of Bordetella bronchiseptica RB50 and its Bvg- phase-locked derivative, RB54 to test and validate a long oligonucleotide B. bronchiseptica microarray

Project description:Based on the genomic sequence and using the freely available software ArrayOligoSelector, a long oligonucleotide B. bronchiseptica microarray was designed and assembled. This long-oligonucleotide microarray was subsequently tested and validated by comparing changes in the global expression profiles between B. bronchiseptica RB50 and its Bvg- phase-locked derivative, RB54.

2007-01-31 | E-MEXP-961 | biostudies-arrayexpress

Transcription profiling of Bordetella bronchiseptica strains along with their phase-locked derivatives, grown at 23C or 37C to investigate the effect of growth temperature

Project description:Using a B. bronchiseptica-specific microarray, we characterized the global gene expression profiles of several B. bronchiseptica strains, along with their phase-locked derivatives, grown at 23oC or 37oC.

2007-10-31 | E-MEXP-1210 | biostudies-arrayexpress

RNA-Seq of BALB/c mouse lung infected with B. bronchiseptica strain RB50, and B. bronchiseptica strain RB50 btrS knockout mutant

Project description:BALB/c and the eosinophil deficient ΔblGATA-1 mice were infected with either B. bronchiseptica strain RB50, or B. bronchiseptica strain RB50 btrS knockout mutant. At day 7 post-inoculation, mice were euthanized and lungs were extracted. RNA from the lungs were analyzed via RNA-Sequencing.

2023-09-22 | E-MTAB-13332 | biostudies-arrayexpress

Transcription profiling by array of Bordetella bronchiseptica strains RB50 and 761 grown in normal or high carbon dioxide conditions

Project description:Comparison of B. bronchiseptica strains RB50 and 761 grown in either atmospheric concentrations of oxygen and carbon dioxide (normal conditions) or in atmospheric levels of oxygen with the addition of 5% carbon dioxide into a sealed incubator (5% CO2 conditions).

2010-10-01 | E-MEXP-2875 | biostudies-arrayexpress

Global transcriptional response of Bordetella bronchiseptica after exposure to blood

Project description:B. bronchiseptica strain RB50 was grown in Stainer-Scholte (SS) broth at 37°C with shaking (220 rpm) overnight. Bacteria were subcultured at a starting OD600 of 0.1 into SS broth and grown at 37°C with shaking (220 rpm) up to an OD600 of 1.0. The cultures were pelleted and bacteria were washed twice with PBS buffer and equal aliquots were resuspended in SS broth (control) or in 100% sheep blood and incubated for 1 hour at 37°C.

2018-10-07 | E-MTAB-6145 | biostudies-arrayexpress

Global transcriptional response of Bordetella bronchiseptica after exposure to serum

2018-10-07 | E-MTAB-6146 | biostudies-arrayexpress