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Transcription profiling of Bordetella bronchiseptica strains 253 and RB50 to investigate adenylate cyclase toxin (ACT) expression

ABSTRACT: Bordetella bronchiseptica is a gram-negative respiratory pathogen that causes a diverse spectrum of respiratory disease in a wide-range of hosts. We sought to determine if strains of B. bronchiseptica differed in virulence using the mouse model of infection. Mean lethal doses (LD50) of different B. bronchiseptica strains varied widely in the murine model. B. bronchiseptica strain 253 had a LD50 that was 10-fold lower than the prototypical and fully sequenced B. bronchiseptica strain RB50. Using whole genomic transcriptome analysis covering 100% of B. bronchisetpctica strain RB50ÃÂÃÂs predicted open reading frames (ORFs), 253 was identified as lacking expression of adenylate cyclase toxin (ACT).

INSTRUMENT(S): GenePix 4000B [Axon Instruments]

ORGANISM(S): Bordetella bronchiseptica

SUBMITTER: Tracy Nicholson

PROVIDER: E-MEXP-1203 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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