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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Cited2 knockout mouse E14.5 fetal liver samples

ABSTRACT: Total RNA (15 ug) isolated from three each of wild type and Cited2-null E14.5 fetal livers using Qiagen RNeasy kit (Valencia, CA) was used to prepare biotinylated cRNA according to the protocol described in Affymetrix Expression Analysis Technical Manual. We hybridized a total of six GeneChip Mouse Genome 430A 2.0 arrays (Affymetrix), three for controls and three for experimental samples.

ORGANISM(S): Mus musculus

SUBMITTER: Shi Gu

PROVIDER: E-MEXP-1240 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cited2, a coactivator of HNF4alpha, is essential for liver development.

Qu Xiaoling X Lam Eric E Doughman Yong-Qiu YQ Chen Yu Y Chou Yu-Ting YT Lam Minh M Turakhia Mona M Dunwoodie Sally L SL Watanabe Michiko M Xu Bing B Duncan Stephen A SA Yang Yu-Chung YC

The EMBO journal 20071011 21

The transcriptional modulator Cited2 is induced by various biological stimuli including hypoxia, cytokines, growth factors, lipopolysaccharide (LPS) and flow shear. In this study, we report that Cited2 is required for mouse fetal liver development. Cited2(-/-) fetal liver displays hypoplasia with higher incidence of cell apoptosis, and exhibits disrupted cell-cell contact, disorganized sinusoidal architecture, as well as impaired lipid metabolism and hepatic gluconeogenesis. Furthermore, we demo ...[more]

PMID: 17932483

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