Transcription profiling of mouse dormant and activated blastocysts

ABSTRACT: Delayed implantation occurs in many mammals, but the underlying mechanisms that direct this process remain largely unknown. In mice, ovariectomy prior to the preimplantation ovarian estrogen secretion on day 4 of pregnancy initiates blastocyst dormancy, which can be maintained for more than 200 days by continued progesterone treatment. An injection of estrogen activates blastocysts rapidly and makes them to implant in the progesterone-primed uterus. Recent establishment of a 60-mer oligo microarray platform, enriched for genes expressed in stem cells and early embryos including preimplantation embryos (7), fulfills this objective. With an optimized labeling reaction for a small amount of RNA with two rounds of cRNA linear amplification, we compared gene expression differences between dorma

ORGANISM(S): Mus musculus

SUBMITTER: Minoru Ko 

PROVIDER: E-MEXP-132 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress