Project description:ATHK1 is a histidine kinase responsive to drought stress.In this experiment, gene expression profile of athk1 knockdown mutant was compared with that of the wild_type under unstressed condition.
Project description:ZmDREB2A is a DREB2-type transcription factor cloned from maize, whose transcript was upregulated by drought, high salt, low temperature and heat stresses. The ZmDREB2A gene possesses two kinds of transcription forms by alternative splicing. Only the functional form was studied to be highly induced by stresses. Transgenic plants overexpressing ZmDREB2A (35S:ZmDREB2A) showed dwarfism and enhanced drought stress tolerance. Microarray analysis of two independent transgenic plants revealed that in addition to genes encoding LEA proteins, some genes related to heat shock and detoxification were also upregulated. Experiments on termotolerance tests of these transgenic plants showed overexpressing ZmDREB2A gene also improved plant tolerance to heat stress.
Project description:To identify genes acting downstream of MAP kinase kinase, and thus map its signal, we used the steroid-inducible overexpression system. The constitutive active form MKK3 (MKK3DD) or MKK4 (MKK4DD) was cloned into the glucocorticoid-inducible vector pTA7002 and transformed using Agrobacterium method into Arabidopsis Col-0 plants. The transgenic plants transformed empty vector was used as control plants (Cont).
Project description:Gene expressions were compared between non-stress Arabidopsis plants (0 hr at low-temperature treatment) and low-temperature treated plants (2 hrs and 10 hrs low-temperature treatment).